Sample Type:|Serum, plasma, tissue homogenates and other biological fluids||Intended Use:|The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFRSF8 in rat serum, plasma, tissue homogenates and other biological fluids.||Sensitivity:|0.135ng/ml||Range:|0.312-20ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8).|No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) and analogues was observed.||Precision:|Intra-Assay: CV<10% |Inter-Assay: CV<12%||Test Principle:|The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFRSF8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNFRSF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFRSF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFRSF8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|96-well strip plate, 1x Plate |Standard, 2x vial|Detection Reagent A, 1×120ul|Detection Reagent B, 1×120ul|TMB Substrate, 1×9ml|Wash Buffer (30X), 1×20ml|Standard Diluent, 1×20ml|Assay Diluent A, 1×12ml|Assay Diluent B, 1×12ml|Stop Solution, 1x6ml||Storage and Stability:|Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. USBio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。