SampleType:|Serum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofMIFinratserum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.||Sensitivity:|0.117ng/ml||Range:|0.312-20ng/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofWide-rangeMacrophageMigrationInhibitoryFactor(MIF).Nosignificantcross-reactivityorinterferencebetweenWide-rangeMacrophageMigrationInhibitoryFactor(MIF)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoMIF.Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoMIF.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainMIF,biotin-conjugatedantibodyand|enzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofMIFinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.||KitComponents:|96-wellstripplate,1xPlate|Standard,2xvial|DetectionReagentA,1×120ul|DetectionReagentB,1×120ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|AssayDiluentB,1×12ml|StopSolution,1x6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological. USBio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。