Serum,Plasma,BIOLOGicalFluids

ThisBioAssay™kitisasandwichELISAforinvitroquantitativemeasurementofAKAP12inhumanserum,plasmaandotherbiologicalfluids

9.375pg/mL

15.625-1000pg/mL

Human

ThisBioAssay™ELISAkitusesSandwich-ELISAasthemethod.ThemicroELISAplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictohumanAKAP12(AKinaseAnchorProtein12).StandardsorsamplesareaddedtotheappropriatemicroELISAplatewellsandcombinedwiththespecificantibody.ThenabiotinylateddetectionantibodyspecificforhumanAKAP12(AKinaseAnchorProtein12)andAvidin-HorserADIshPeroxidase(HRP)conjugateareaddedtoeachmicroplatewellsuccessivelyandincubated.Freecomponentsarewashedaway.Thesubstratesolutionisaddedtoeachwell.OnlythosewellsthatcontainhumanAKAP12(AKinaseAnchorProtein12),biotinylateddetectionantibodyandAvidin-HRPconjugatewillappearblueincolor.Theenzyme-substratereactionisterminatedbyaddingStopSolutionandthecolorturnsyellow.Theopticaldensity(OD)ismeasuredspectrophotometricallyatawavelengthof450nm±2nm.TheODvalueisproportionaltotheconcentrationofhumanAKAP12(AKinaseAnchorProtein12).TheconcentrationofhumanAKAP12(AKinaseAnchorProtein12)insamplescanbecalculatedbycomparingtheODofthesamplestothestandardcurve.

MicrotiterStrips,8x12wellsStandard,2vialsSample/StandardDiluentBuffer,1x20mlAntibody-Biotin,1x120ulAntibody-BiotinDiluent,1x10mlHRPConjugate,1x120ulHRPConjugateDiluent,1x10mlSubstrateReagent,1x10mlStopSolution,1x10mlWashBuffer(25X),1x30ml

Unusedkitisstablefor6monthsafterreceipt. StoreMicrotiterStrips,Standard,andAntibody-Biotinat4°Cifkitwillbeusedwithin30days;otherwisestoreat-20°Cuntilexpirationdate.Storetherestofthecomponentsat4°C.ProtectHRPConjugateandSubstrateReagentfromlight.Formaximumrecovery,centrifugetheoriginalvialsafterthawingandpriortoremovingthecap.