TransfectionReagent(invivo)ishighlyeffectiveforDNAplasmiddeliveryinvivo,whilenotinducinganinflammatoryresponse.TurboFectinvivohasbeenassessedforplasmidDNAdeliveryinbothmiceandratsandissuitableforDNAadmiNISTrationviavariousroutes,includingintravenous,intraperitoneal,andintratumoralinjection.TheamountofDNAandsuggestedinjectionvolumewilldependontheexperimentalanimalandtherouteofadministrationaswellasthetargetedtissueororganandtheexpressionvector.||TransfectionReagent(invivo)isasterilesolutionofacationicpolymerinwater.Thepolymerformscompact,stable,positivelychargedcomplexeswithDNA.ThesecomplexesprotectDNAfromdegradationandfacilitategenedeliveryinvivo.ReagentcausesnodetectableinflamatoryresponseandissuitableforDNAadministrationviavariousroutes,includingintravenous,intraperitoneal,intratumoralinjection,etc.||TransfectionReagent(invivo)for20transfectionsusing50ugDNA.||ReagentsRequiredbutnotSupplied:|Sterile5%glucosesolutionfordilutionofDNA.||GeneralConsiderations:||DNAQualityRequirements:|DNAqualityiscriticalforsuccessfultransfection.AnA260/A280ratioof1.8orhigherisrecommended.DNAshouldbesterileandfreeofanycontaminantssuchasendotoxins.||ChoiceofPromoter:|Highgeneexpressiondependsonboththepromoterunderwhichthegeneofinterestisexpressedandthetargetedtissue/organ.Cytomegalovirus(CMV)promoterisbestknownforhighgeneexpressioninawidevarietyofcells.SomeresearcherspreferLTR,simianvirus(SV40)orRoussarcomavirus(RSV)promoters.||TransfectionReagent/DNARatio:|ThevolumeoftransfectionreagentuseddependsontheamountofDNA,transgeneandanimalstobetransfected.Theratiospresentedintheprotocolsbelowarestartingamountsandcanbefurtheroptimizedforbestresults.||AmountofDNAandsuggestedinjectionvolume:|TheamountofDNAandsuggestedinjectionvolumedependontheexperimentalanimalandtherouteofadministration(seeprotocol)aswellasthetargetedtissueororganandtheexpressionvector.TopreventprecipitationofTransfectionReagent/DNAcomplexes,thefinalconcentrationofDNAintheinjectionmixshouldnotexceed0.5ug/ul.||QualityControl:|TransfectionefficiencywastestedonBALB/cfemalemice(7-8weeksold)using50ugFireflyluciferasecodingplasmidand6ulofinvivoTransfectionreagentspermouse.ThedeliveryofthetransgenewasevaluatedbymeasurementofFireflyluciferaseactivityintotalproteinextractedfromthelungsoftransfectedanimals.100%oftestedmiceexpressedtheFireflyluciferase.||StorageandStABIlity:|Maybestoredat4°C.Forlong-termstorage,aliquotandstoreat4°C.Donotfreeze.Aliquotsarestablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap. USBio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。