BacterialDNAisknowntoinduceacuteinflammatoryresponses.BacterialDNAactsasapathogen-associatedmolecularpatternbyvirtueofa20-foldgreaterfrequencyofunmethylatedCGdinucleotidesfoundinmicrobialDNAversusvertebrateDNA.Cellularactivationbydeoxy-cytidylate-phosphate-deoxy-guanylate(CpG)-DNAoccursviatheToll/IL-1Rsignalpathway.TLR9appearstobeamajorcomponentoftheCpG-DNAreceptor,actingbydirectbindingtoCpG-DNA.Thesyntheticoligodeoxynucleotides(ODN)containtheproperCpG-DNAmotifmimicingtheimmunostimulatoryeffectsofbacterialDNA.ThehumanoptimalCpGmotifGTCGTTdiffersfromtheoptimalmouseCpGmotifGACGTT.NonCpG-DNAhasbeenshowntocompetewithCpG-DNAininvitrostimulationexperiments.||Source:|ThismouseCpGisa20-merODNhasthefollowingsequence:5'-tccatgacgttcctgatgct-3'.Regularlettersrepresentphosphorothioratelinkage.||Applications:|SuitableforuseforBIOLOGicalassaysinvitrotoactivatemurinecells.Otherapplicationsnottested.||RecommendedDilutions:|InvitroStimulation:0.01-10uM|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Lyophilizedpowdermaybestoredat-20°C.Stablefor12monthsat-20°C.ReconstitutewithdH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer. USBio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。