Description

RunBlue Features & BenefitsNovel optimised gel-buffer system - High resolution and sharp bandsExtra resolving length - Increased separationUnique homogeneous polymerization - Increased consistency and no residual free acrylamideExtra Wells - Run up to 12 or 17 samples simultaneouslyDeep and wide wells - Load up to 35 µl (12 well) or 20 ul (17 well)Standard format - Compatible with 10x10cm protein gel tanks such as NuPAGE® XCell SureLock Mini-CellProtruding teeth - No well contamination in case of overloadingResilient gel matrix - Tear-proof gel and risk-free handlingComb-free - No potentially broken or damaged teethStrip-free - No potentially ruined Western BlotsClipped cassette - Fast and gentle openingNeutral buffer and high chemical stability - shelf life of 2 years at 4ºC and 6 months at RTRunBlue PerformanceRunBlue SDS Protein Gels take advantage of Expedeon’s new methods and innovations to the gel making process to increase resolution and reproducibility of separated protein bands. RunBlue SDS Protein Gels are based on a novel gel buffer system which provides a separation profile similar to Nupage® Bis-Tris gels, but with enhanced separation of higher molecular weight proteins and better overall resolution. RunBlue Protein Gel’s proprietary polymerization process results in more uniform gels between batches, with decreased variability and improved repeatability of results.Usability EnhancementsIn addition to their high resolution separation of protein all RunBlue Protein Gels have a number of enhancements to improve and streamline usability. RunBlue Protein Gels are up to 10 times stronger than conventional hand-cast gels and can stand up to repeated handling. This practically eliminates the risk of torn and unusable gels encountered during normal gel manipulation. More lanes than standard gels allows more proteins to be run and wide, deep wells allow higher volumes to be loaded.Cassettes have no comb or tape to remove and no excess gel to trim, all of which improves the speed and efficiency of the loading and running process. Wells are outlined and numbered for quicker sample loading and the cassette is labeled with gel type, percentage and a unique lot number for traceability. Well teeth protrude above the cassette to prevent sample contamination between wells in case of overloading.Flexible UsageThe RunBlue SDS Protein Gel 10x10cm cassettes are designed to fit a number of gel tanks such as Invitrogen® XCell SureLock™ equipment, Hoeffer, and Owl tanks. RunBlue SDS Protein Gels are highly optimised for a TEO-Tricine buffer system and must be run with this buffer system to achieve good results. TEO-Tricine buffer is available as a premixed 20X solution, or a homemade version can be mixed using the available recipe. The samples must be prepared with a TEO-Cl based sample buffer which is available as a premade 4X solution, or can be homemade with the freely available recipe.RunBlue Protein Gels have an extended shelf life of 2 years at 4ºC which is ideal for large stocks or infrequent use. They also have a 6 month shelf life at room temperature which is convenient when fridge space is limited.Improved SafetyPolyacrylamide gels are based on the polymerization of liquid acrylamide and bis-acrylamide. The polymerization is usually induced by the addition of a source of free radicals and a stabilizer (usually APS & TEMED) which induces cross link formation between acrylamide and bis-acrylamide monomers. This localized chemical induction results in heterogeneous polymerization across the gel due to acrylamide concentration gradients being generated, and can lead to the presence of residual free acrylamide at the end of the polymerization.Expedeon has developed a unique physical induction technology which ensure homogenous polymerization across the whole gel and no residual free acrylamide after the end of the polymerization. Numerous studies have shown the acrylamide monomer is a neurotoxin and possible carcinogen*. Acrylamide can be inhaled as a dry powder, evaporate from liquid solution and sublimate from solid gels if not fully polymerized. RunBlue gels are polymerized in a unique homogenous manner that results in a consistent gel with minimal or no residue after casting.

Application Notes

These products are packs of 10 gels.

Novus Biologicals 美国Novus Biologicals公司是一家抗体专业公司，生产用于研究的单克隆和多克隆抗体，包含5万多种一抗和近1000种二抗，产品涉及神经生物学，细胞凋亡，肿瘤，DNA修复，干细胞标记，信号转导等多个研究方向。多年的抗体生产服务经验，使novus得以引领生物科学发展趋势，并可随时为生物科研工作者提供最新和最广泛的产品。Novus的愿景是通过开发和销售在前沿科学的独特产品来推动科学发现。Novus组织了商业化机制，致力于为生物研究界提供独特的生物材料。Novus还持续关注科学趋势，并为新的研究提供必要的材料。Novus的产品广泛使用于机构和商业研究，并在推动生物学研究中起着重要的。 Novus Biologicals is a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.[1] The company develops, licenses and markets research materials to scientists in academic, corporate and government laboratories. Novus, like many others companies such as Abcam, Covance, Proteintech Group, EnCor Biotechnology and Millipore Corporation, arose to answer a call for research grade antibodies for the scientific market.Founded by Karen Padgett in 1996, Novus Biologicals has sought to partner with leading scientific researchers and third-party providers to develop an extensive range of more than 180,000 products.[2] The main products are antibodies, proteins, peptides, RNAi, cell lysates, isotype controls, slides, and kits. Padgett is company president and CEO, and has been active both in the academic and local Colorado communities. In 2008, Novus became a limited liability company when Mainsail Partners invested in the company.[4] Novus Biologicals has reported double digit revenue growth every year since its inception, has been selected six years in a row to the Inc. (magazine) 5000 fastest-growing private companies in America,[5] and was on the 2009 list of Colorado Companies to Watch.[6]In 2014 major antibody and reagent supplier Bio-Techne, acquired Novus Biologicals for $60 million.[7] The acquisition was meant to provide scientists and doctors a broader selection of products in one location. 产品列表： Novus-Biologicals SP4385P MC1 Receptor Antibody 0.05mg Novus-Biologicals PA184X Defensin beta 3 Protein 0.02mg Novus-Biologicals NSB998 PKA 2 beta [phospho Ser114] Antibody 0.1ml Novus-Biologicals NSB992 cAMP Protein Kinase Catalytic subunit beta [phospho Ser338] Antibody 0.05ml Novus-Biologicals NSB988 cAMP Protein Kinase Catalytic subunit alpha [phospho Thr197] Antibody 0.1ml Novus-Biologicals NSB979 PKC theta [phospho Ser695] Antibody 0.1ml Novus-Biologicals NSB978 PKC eta [phospho Ser674] Antibody 0.1ml Novus-Biologicals NSB977 PKC epsilon [phospho Ser729] Antibody 0.1ml Novus-Biologicals NSB976 PKC delta [phospho Ser664] Antibody 0.1ml Novus-Biologicals NSB975 PKC gamma [phospho Thr674] Antibody 0.1ml Novus-Biologicals NSB970 PKC theta [phospho Ser676] Antibody 0.1ml Novus-Biologicals NSB969 PKC eta [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB968 PKC iota [phospho Thr555/Thr563] Antibody 0.1ml Novus-Biologicals NSB966 PKC delta [phospho Ser645] Antibody 0.1ml Novus-Biologicals NSB965 PKC gamma [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB964 PKC beta [phospho Thr641] Antibody 0.1ml Novus-Biologicals NSB963 PKC beta [phospho Thr642] Antibody 0.1ml Novus-Biologicals NSB962 PKC alpha [phospho Thr638] Antibody 0.1ml Novus-Biologicals NSB961 PKC mu [phospho Ser738/Ser742] Antibody 0.1ml Novus-Biologicals NSB960 PKC theta [phospho Thr538] Antibody 0.1ml Novus-Biologicals NSB956 PKC gamma [phospho Thr514] Antibody 0.1ml Novus-Biologicals NSB955 PKC beta [phospho Thr500] Antibody 0.1ml Novus-Biologicals NSB948 GATA4 [phospho Ser105] Antibody 0.1ml Novus-Biologicals NSB944 NFAT1 [phospho Ser54] Antibody 0.1ml Novus-Biologicals NSB942 PAK1/2/3 [phospho Thr423] Antibody 0.1ml Novus-Biologicals NSB940 PAK1/2/3 [phospho Ser141] Antibody 0.1ml Novus-Biologicals NSB928 RSK1 [phospho Ser380] Antibody 0.1ml Novus-Biologicals NSB926 RSK1 [phospho Ser363] Antibody 0.1ml Novus-Biologicals NSB924 RSK1 [phospho Ser221] Antibody 0.1ml Novus-Biologicals NSB923 RPS6 [phospho Ser244/Ser247] Antibody 0.1ml Novus-Biologicals NSB922 RPS6 [phospho Ser235/Ser236] Antibody 0.1ml Novus-Biologicals NSB921 RPS6 [phospho Ser236] Antibody 0.1ml Novus-Biologicals NSB918 S6K [phospho Thr229] Antibody 0.1ml Novus-Biologicals NSB912 Src [phospho Tyr522] Antibody 0.1ml Novus-Biologicals NSB910 Hck [phospho Tyr207/Ser211] Antibody 0.1ml Novus-Biologicals NSB909 DAB1 [phospho Ser491] Antibody 0.1ml Novus-Biologicals NSB908 DAB1 [phospho Tyr220] Antibody 0.1ml Novus-Biologicals NSB906 DAB1 [phospho Tyr198] Antibody 0.1ml Novus-Biologicals NSB896 Met (c-Met) [phospho Tyr1365] Antibody 0.1ml Novus-Biologicals NSB892 Met (c-Met) [phospho Tyr1349] Antibody 0.1ml