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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
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https://www.ebiomall.com
商品描述
RecombinantHumanTGF-beta1ProteinSummary
| AdditionalInformation | ANewTGF-beta1isAvailable!ThenewproteinisHek293expressed! |
| DetailsofFunctionality | |
| Source | ChineseHamsterOvarycellline,CHO-derivedhumanTGF-beta1proteinAla279-Ser390 |
| Accession# | P01137 |
| N-terminalSequence | Ala279 |
| Structure/Form | Disulfide-linkedhomodimer |
| Protein/PeptideType | RecombinantProteins |
| Gene | TGFB1 |
| Purity | >97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining. |
| EndotoxinNote | <0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.10> |
Applications/Dilutions
| TheoreticalMW | 12.8kDa(monomer).Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors. | |
| SDS-PAGE | 12kDa,reducingconditions24kDa,non-reducingconditions | |
| Publications |
|
Packaging,Storage&Formulations
| Storage | Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
|
| Buffer | Lyophilizedfroma0.2μmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein. |
| Purity | >97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining. |
| ReconstitutionInstructions | PurifiedrecombinanthumanTGF-beta1isanextremelyhydrophobicproteinthatadheresstronglytosurfaces.Toensurerecovery,reconstituteat20μg/mLinsterile4mMHClcontaining1mg/mLhumanorbovineserumalbumin. |
Notes
ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.
AlternateNamesforRecombinantHumanTGF-beta1Protein
- CEDLAP
- DPD1
- latency-associatedpeptide
- TGFbeta1
- TGFB
- TGFB1
- TGF-beta1protein
- TGFbeta1
- TGF-beta1
- TGFbeta
- TGF-beta-1
- transforminggrowthfactorbeta-1
- transforminggrowthfactor,beta1
Background
TGF-beta1(transforminggrowthfactorbeta1)isoneofthreecloselyrelatedmammalianmembersofthelargeTGF-betasuperfamilythatshareacharacteristiccystineknotstructure(1‑7).TGF-beta1,-2and-3arehighlypleiotropiccytokinesthatareproposedtoactascellularswitchesthatregulateprocessessuchasimmunefunction,proliferationandepithelial-mesenchymaltransition(1‑4).EachTGF-betaisoformhassomenon‑redundantfunctions;forTGF-beta1,micewithtargeteddeletionshowdefectsinhematopoiesisandendothelialdifferentiation,anddieofoverwhelminginflammation(2).HumanTGF‑beta1CDNAencodesa390aminoacid(aa)precursorthatcontainsa29aasignalpeptideanda361aaproprotein(8).Afurin‑likeconvertaseprocessestheproproteintogenerateanN‑terminal249aalatency‑associatedpeptide(LAP)andaC‑terminal112aamatureTGF‑beta1(8,9).Disulfide‑linkedhomodimersofLAPandTGF‑beta1remainnon‑covalentlyassociatedaftersecretion,formingthesmalllatentTGF‑beta1complex(8‑10).CovalentlinkageofLAPtooneofthreelatentTGF‑betabindingproteins(LTBPs)createsalargelatentcomplexthatmayinteractwiththeextracellularmatrix(9,10).TGF‑betaisactivatedfromlatencybypathwaysthatincludeactionsoftheproteaseplasmin,matrixmetalloproteases,thrombospondin1andasubsetofintegrins(10).MaturehumanTGF‑beta1shares100%aaidentitywithpig,dogandcowTGF‑beta1,and99%aaidentitywithmouse,ratandhorseTGF‑beta1.Itdemonstratescross‑speciesactivity(1).TGF‑beta1signalingbeginswithhigh‑affinitybindingtoatypeIIser/thrkinasereceptortermedTGF‑betaRII.Thisreceptorthenphosphorylatesandactivatesasecondser/thrkinasereceptor,TGF‑betaRI(alsocalledactivinreceptor‑likekinase(ALK)‑5),oralternatively,ALK‑1.ThiscomplexphosphorylatesandactivatesSmadproteinsthatregulatetranscription(3,11,12).Contributionsoftheaccessoryreceptorsbetaglycan(alsoknownasTGF‑betaRIII)andendoglin,oruseofSmad‑independentsignalingpathways,allowfordisparateactionsobservedinresponsetoTGF‑betaindifferentcontexts(11).
- Derynck,R.andK.Miyazono(2008)ColdSpringHarborLaboratoryPress,29.
- Dunker,N.andK.Krieglstein(2000)Eur.J.Biochem.267:6982.
- Wahl,S.M.(2006)Immunol.Rev.213:213.
- Chang,H.etal.(2002)Endocr.Rev.23:787.
- Lin,J.S.etal.(2006)Reproduction132:179.
- Hinck,A.P.etal.(1996)Biochemistry35:8517.
- Mittl,P.R.E.etal.(1996)ProteinSci.5:1261.
- Derynck,R.etal.(1985)Nature316:701.
- Miyazono,K.etal.(1988)J.Biol.Chem.263:6407.
- Oklu,R.andR.Hesketh(2000)Biochem.J.352:601.
- deCaestecker,M.etal.(2004)CytokineGrowthFactorRev.15:1.
- Zuniga,J.E.etal.(2005)J.Mol.Biol.354:1052.
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美国Novus Biologicals公司是一家抗体专业公司,生产用于研究的单克隆和多克隆抗体,包含5万多种一抗和近1000种二抗,产品涉及神经生物学,细胞凋亡,肿瘤,DNA修复,干细胞标记,信号转导等多个研究方向。多年的抗体生产服务经验,使novus得以引领生物科学发展趋势,并可随时为生物科研工作者提供最新和最广泛的产品。Novus的愿景是通过开发和销售在前沿科学的独特产品来推动科学发现。Novus组织了商业化机制,致力于为生物研究界提供独特的生物材料。Novus还持续关注科学趋势,并为新的研究提供必要的材料。Novus的产品广泛使用于机构和商业研究,并在推动生物学研究中起着重要的。
Novus Biologicals is a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.[1] The company develops, licenses and markets research materials to scientists in academic, corporate and government laboratories. Novus, like many others companies such as Abcam, Covance, Proteintech Group, EnCor Biotechnology and Millipore Corporation, arose to answer a call for research grade antibodies for the scientific market.Founded by Karen Padgett in 1996, Novus Biologicals has sought to partner with leading scientific researchers and third-party providers to develop an extensive range of more than 180,000 products.[2] The main products are antibodies, proteins, peptides, RNAi, cell lysates, isotype controls, slides, and kits. Padgett is company president and CEO, and has been active both in the academic and local Colorado communities.
In 2008, Novus became a limited liability company when Mainsail Partners invested in the company.[4] Novus Biologicals has reported double digit revenue growth every year since its inception, has been selected six years in a row to the Inc. (magazine) 5000 fastest-growing private companies in America,[5] and was on the 2009 list of Colorado Companies to Watch.[6]In 2014 major antibody and reagent supplier Bio-Techne, acquired Novus Biologicals for $60 million.[7] The acquisition was meant to provide scientists and doctors a broader selection of products in one location.
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![IL-6 [Unconjugated] IL-6 [Unconjugated]](images/novusbio/novus_guarantee.png)



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![Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/ml.](http://images.novusbio.com/images2/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg)
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![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
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