TheonlycommerciallyavailableELISAassay

IBLInternationalprovidesareliableandfastimmunoassayforthedeterminationofthemainmetaboliteofhistamine,1-methylhistamine(N-methylhistamine),invariousBIOLOGicalfluids.

Extensiveresearchhasshownthathistamine[2-(4-imidazolyl)-ethylamine]istheprinciplemediatorofmultiplebiologicalprocessesincludingneuromodulation,inflammation,gastricacidsecretionandtheregulationofimmunefunction.Itismainlystoredinthemetachromaticgranulaofmastcellsandthebasophilicleukocytes,andreleaseduponstimulation.

HistamineispredominantlymetabolizedviaN-methytransfereseto1-methylhistamine,whichisfurtherprocessedtoN-methylimidazoleaceticacid,whiletheremainderismetabolizedbydiamineoxidasetoimidazoleaceticacid(Fig.1).Theurinary1-methylhistaminelevelcorrelateswellwiththehistaminelevelsinplasmaandurine^(1,2),andismorestablethanhistamineinplasma.

Thedeterminationofurinary1-methylhistaminefindsitapplicationprimarilyinthediagnosisandmonitoringofmast-celldisorders,includingmastocytosis,anaphylaxis,andotherseveresystemicallergicreactions.

Uniquefeatures

• TheonlycommerciallyavailableELISA
• Sensitivity:urine:2.5ng/mL,plasmaandcellculture:0.13ng/mL
• CalibratedtoLC-MS/MS(Fig.2)
• Totalassaytimeisonly2hours
• Alsoapplicableforplasmaandcellculturesamples

Literature
1.HermannK.etal.Measurementandcharacterizationofhistamineandmethylhistamineinhumanurineunderhistamine-richandhistamine-poordiets.IntArchAllergyImmunol.1993;101(1):13-9.
2.BelicA.etal.Interdependenceofhistamineandmethylhistaminekinetics:modellingandsimulationapproach.ComputBiolMed.1999Nov;29(6):361-75.
3.MaintzL.etal.Histamineandhistamineintolerance.AmJClinNutr2007;85:1185–96.

DistributedbyIBLInternational.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside. iBL的Tecan由四名工程师于1980年在瑞士Hombrechtikon村成立，旨在开发测量，分析和实验室自动化设备。最初的办公室位于经过翻新的农舍中，这是首席执行官兼创始人亨氏·阿普兰纳普（Heinz Abplanalp）的家庭住宅。