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M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.
Source of ProteinA recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.
Supplied in50 mM Tris-HCl150 mM NaCl0.1 mM EDTA1 mM DTT0.1% NP-40 Alternative50% glycerolpH 7.6 @ 25°C
Supplied WithB7040 (10X M MuLV RT Buffer)
10X M-MuLV RT Buffer (B7040):500 mM Tris-HCl750 mM KCl30 mM MgCl2100 mM DTTpH 8.3 @ 25°C
Unit Definition1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
酶科技公司产品包括八类:超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。 Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation. EnzScriptPhoenix Hot Start Taq产品列表: 品牌 名称 1 N2070-10-L 2 N2050-10-L 100 µmol Enzymatics 100 mM 75µmol dNTP Solution Set75 µmol Enzymatics 100 mM dATP Solution75 µmol Enzymatics 100µmol dNTP Solution Mix100 µmol Enzymatics 10x Uracil Cleavage System750 Reactions Enzymatics 2x HiFi PCR Master Mix1 rxn Enzymatics 5x ER/A-Tailing Enzyme Mix24 reactions Enzymatics 5X WGS Fragmentation Mix24 Reactions Enzymatics Bst X DNA Polymerase40,000 U Enzymatics DNA Polymerase I5,000 U Enzymatics E. coli DNA Ligase2,500 U Enzymatics E. coli fpg4,000 U Enzymatics E. coli Pyrophosphatase50 U Enzymatics E. coli Single-Stranded DNA Binding Protein1.5 mg

