E. coli DNA ligase catalyzes the phosphodiester bond formation between an adjacent 5′ phosphate and a 3′ hydroxyl of DNA ends, requiring NAD⁺ and Mg²⁺ as cofactors. Ligation of blunt ended DNA is extremely inefficient relative to cohesive DNA end ligation and nick sealing.

Source of ProteinThe gene encoding E. coli DNA Ligase expressed from a plasmid in E. coli.

Supplied in10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied With:B6090 (10X E.coli DNA Ligase Reaction Buffer)

10X E.coli DNA Ligase Reaction Buffer (B6090):300 mM Tris-HCl40 mM MgCl₂260 µM NAD10 mM DTT0.5 mg/mL BSApH 8.0 @ 25°C

Unit Definition1 unit is defined as the amount of E.coli DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 25°C.

酶科技公司产品包括八类：超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。 Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation. EnzScriptPhoenix Hot Start Taq产品列表： 品牌 名称 1 N2070-10-L   2 N2050-10-L 100 µmol Enzymatics 100 mM 75µmol dNTP Solution Set75 µmol Enzymatics 100 mM dATP Solution75 µmol Enzymatics 100µmol dNTP Solution Mix100 µmol Enzymatics 10x Uracil Cleavage System750 Reactions Enzymatics 2x HiFi PCR Master Mix1 rxn Enzymatics 5x ER/A-Tailing Enzyme Mix24 reactions Enzymatics 5X WGS Fragmentation Mix24 Reactions Enzymatics Bst X DNA Polymerase40,000 U Enzymatics DNA Polymerase I5,000 U Enzymatics E. coli DNA Ligase2,500 U Enzymatics E. coli fpg4,000 U Enzymatics E. coli Pyrophosphatase50 U Enzymatics E. coli Single-Stranded DNA Binding Protein1.5 mg