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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Technical Documents
Description
Details
Intellectual Property
Dilution/Coupling Data
References
Technical Documents
Description
5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat.Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions.Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction.TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection.Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup.A quick treatment with TBAF removes the TIPS protecting group.
Details
Usage
- Coupling: 3 minute coupling time recommended.
- Deprotection: Ammonium hydroxide for 2 hours at 55°C or as required by nucleobases.|TIPS removal: After deprotection, dry down oligo and take up in 0.5 mL DMF.Add 0.1 mL TBAF. Vortex and keep at 45°C for 15 minutes.Quench with 0.5 mL 2 M TEAA and desalt on Glen Gel-Pak™ column or equivalent.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30�C, dry |
| Stability | 1-2 days |
Intellectual Property
baseclick GmbH has been granted the following patents (1-3) besides its further patent applications (4-5). |1. WO 2006/117161 (New labelling strategies for the sensitive detection of analytes)|2. WO 2008/952775 (Click chemistry for the production of reporter molecules)|3. WO 2010/115957 (Click Chemistry on heterogeneous catalysts)|4. PCT/EP 2013/064610 (Anandamide-modified nucleic molecules)|5. PCT/EP 2015/056007 (Self-assembly of DNA Origami: a diagnostic tool)|baseclick GmbH holds a worldwide exclusive license for granted patent application|WO 03/101972 (Copper-catalysed ligation of azides and acetylenes for the nucleic acid field) in the area of diagnostics and research. As Glen Research and baseclick are partners, Glen Research is now able to help in sublicensing this outstanding technology.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1544-02 | 0.25 g | .25grams | 2.52 | 70.67 | 42.4 | 26.5 | 19.27 | 14.13 | 3.53 |
| 10-1544-90 | 100 µmol | .099grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1544-95 | 50 µmol | .05grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1544-02 | 0.25 g | .25grams | 3.76 | 0.07 | 68.8 | 43 | 31.27 | 4.3 |
| 10-1544-90 | 100 µmol | .099grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1544-95 | 50 µmol | .05grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
References
REFERENCE(S)| 1 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064; V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.| 2 C. J. Burrows, J. G. Muller, Chem. Rev. 1998, 98, 1109 â 1151.| 3 T. R. Chan, R. Hilgraf, K. B. Sharpless, V. V. Fokin, Org. Lett. 2004, 6, 2853 â 2855.| 4 J. Gierlich, G. A. Burley, P. M. E. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639-3642. F. Seela, V. R. Sirivolu, Chem. Biodiversity 2006, 3, 509-514.| 5 P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chem. 2008, 120, 3491â3493; Angew. Chem. Int. Ed. 2008, 47, 3442â 3444.| 6 P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chem. Int. Ed. 2008, 47, 8350-8358.
Related Products
Related Products
C8-Alkyne-dT-CE PhosphoramiditeCatalog #: 10-1540
C8-TMS-Alkyne-dT-CE PhosphoramiditeCatalog #: 10-1545
C8-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1543
C8-TMS-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1542
C8-TIPS-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1541
