Description	GeneExpresso™PlusDNAInVitroTranfectionReagentisenhancedversionofGeneExpresso™DNAInVitroTransfectionReagent.GeneExpresso™Plusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonsomemammaliancellsincomparisonofitspreviousversion.GeneExpresso™PluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GeneExpresso™Plusreagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates.
Advantage	Cell-dependent3~20timeshigherefficiency Topchoiceforhard-to-transfectcells EquallygoodforverylongDNAs(upto2.0mb) Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes Exceptionalhighlevelsofrecombinantproteinproduction ComparisonsofTransfectionEfficiencyofGeneExpresso™PlusInVitroDNATransfectionReagentwiththeLeADIngBrandNameProducts GeneExpresso™Plusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGeneExpresso™PlusandL2Krespectivelypermanufacturer'sprotocols.Theefficiencywaschecked48hoursposttransfection. LeftPanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells. Rightpanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithtranfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry. Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGeneExpresso™PlusandLipofectamineLTXwerequantifiedtobe2.1x106and9.3x106tu/mlrespectively.
Protocol	DNATransfectionProtocol
StorageConditions	Uponarrivalstorethisproductat-20oCor4oC.Ifstoredproperly,theproductwillbestablefor12monthsorlonger.Productshippedatambienttemperature.
Discount	NewCustomer:Pleaseenterdiscountcode98Onlytoreceiveupto$100discount(1ml). ReturnCustomer:Volumediscountorquotationisavailable(sendemailtocrm@Excellgen.com) 100µltrialsampleisfornewcustomersonly
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