Description	RecombinantBstDNAPolymerase(largefragment)has5’->3’polymeraseactivity,butlacks5’->3’exonucleaseactivity.ThisenzymecanbeusedforandrapidamplificationoftraceamountsofDNAtemplatesforsequencing.
Applications	DNAsequencingthroughhighGCregions RapidamplificationoftraceamountsofDNAtemplates NextGenerationsequencing
Source	ArecombinantE.colistraincarryingthelargefragmentofDNApolymerasegenefromBacillusstearoThermophilus
UnitDefinition	Oneunitisdefinedastheamountofpolymeraserequiredtocatalyzetheincorporationof10nmolofdNTPintoacid-insolublematerialin30minutesat65°C.
Components	BstDNAPolymerase:8,000U/mlin10mMTris-HCl,50mMKCl,1.0mMDithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%Glycerol,pH7.5@25°C 10XReactionBuffer:200mMTris-HCl,100mM(NH4)2SO4,100mMKCl,20mMMgSO4,and1.0%TritonX-100,pH8.8@25°C
QualityControl	Theabsenceofendonuclease,nickingactivityandexonucleaseactivityisconfirmedbyappropriatequalitytests.
StorageCondition	-20°C

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