Description	Terminaldeoxynucleotidyltransferase(TdT)isatemplate-independentDNApolymerasethatcatalyzestheadditionofdeoxynucleotidestothe3'hydroxylterminusofsingleordoublestrandedDNAmolecules.Thepresenceof1mMCo2+stimulatesthetailingofthe3'-endsofDNAfragments.
Applications	Additionofhomopolymertailstothe3'endsofDNA Labelingthe3'endsofDNAwithmodifiednucleotides(e.g.,ddNTP,DIG-dUTP) TUNELassay(insitulocalizationofapoptosis) TdTdependentPCR
Source	ArecombinantE.colistraincarryinganengineeredhumanterminaldeoxynucleotidyltransferasegene
UnitDefinition	1unitisdefinedastheamountofpolymeraserequiredtoconvert1nmolofdATPsintoacidinsolublematerialin1hourat37°C.
Components	TerminaldeoxynucleotidylTransferase:20,000U/mlin50mMKPO4,100mMNaCl,1.0mMDTT,0.1mMEDTA,50%Glycerol,0.1%TritonX-100,pH7.3@25°C 10XReactionBuffer:200mMTris-Acetate,500mMPotassiumAcetate,100mMMagnesiumAcetate,10mMDTT,pH7.9@25°C 2.5mMCoCl2
StorageCondition	-20°C

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