Description	ThermostableTaqDNALigasecatalyzestheformationofaphosphodiesterbondinduplexDNAcontainingadjacent5'-phosphoryland3'-hydroxyltermini,usingNAD+asacofactor.
Applications	LigationofDNAfragmentsatelevatedtemperatures(45°C-65°C). LigaseChainReaction. GibsonAssemblyofDNAfragments(GeneSynthsis,joningmultiplelargeDNAfragments).
Source	PurifiedfromarecombinantE.colistraincarryingtheclonedTaqDNALigasegene.
Components	TaqDNALigase,40U/µlinstoragebuffer:10mMTris-HCl,50mMKCl,1mMDithiothreitol,0.1mMEDTA,0.1%Tween-2050%Glycerol,pH7.5@25°C. 10XTaqDNALigationBuffer:200mMTris-HCI,250mMKCl,100mMMgCl2,5mMNAD,0.1%TritonX-100,pH7.6@25°C
UnitDefinition	1unitisdefinedastheamountofTaqDNALigaserequiredtojoin50%of1µgofthe12-basecohesiveendsofLamBDaDNAcutwithHindIIIin50µl1XTaqDNALigaseBufferfollowinga10minuteincubationat45°C.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

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