Description	T4PolynucleotideKinase(PNK)catalyzesthetransferoftheterminalgamma-phosphatefromATPtothe5′-OHgroupofsingle-anddouble-strandedDNA,RNA,oligonucleotidesandnucleoside3′-monophosphatemolecules.InthepresenceofADPT4PolynucleotideKinasealsoexhibits5'-phosphataseactivityandcatalyzestheexchangeofphosphategroupsbetween5'-P-oligo-polynucleotidesandATP.
Applications	End-labelingDNAorRNAforprobesandDNAsequencing Additionof5´-phosphatestooligonucleotidestoallowsubsequentligation Removalof3´-phosphorylgroups
Source	ArecombinantE.colistraincarryingT4phagepseTgeneforpolynucleotidekinase.
UnitDefinition	Oneunitisdefinedastheamountofenzymecatalyzingtheincorporationof1nmolof[32P](ATPdonor)in30minutesat37°Cin1XT4PolynucleotideKinaseReactionBuffer.
Components	T4PolynucleotideKinase:10,000U/mlin10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%Glycerol,0.1μMATP,pH7.4@25°C 10XReactionBuffer:700mMTris-HCl,100mMMgCl2,50mMDTT,pH7.6@25°C
QualityControl	Theabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.Functionallytestedforlabeling5'-terminiofDNA.
StorageCondition	-20°C

Excellgen癌蛋白产品名称-目录 ＃价钱Lelch样ECH相关蛋白1，KEAP1RP-71$ 250.00p53，重组野生型人p53蛋白RP-100$ 300.00p53 [1-363] C端缺失，重组蛋白RP-101$ 300.00p53 [1-342] C端缺失，重组蛋白RP-102$ 300.00pRB（视网膜母细胞瘤蛋白）RP-104$ 338.00威尔姆肿瘤抑制因子1（+ KTS）RP-105$ 338.00VHL（von Hippel-Lindau肿瘤抑制蛋白）RP-106$ 290.00C-Fos（原癌基因）RP-107$ 280.00c-jun（原癌基因）RP-108$ 280.00c-myc（原癌基因）RP-109$ 280.00BRCA1（肿瘤抑制蛋白和转录因子）RP-110$ 340.00威尔姆肿瘤抑制因子1（-KTS）RP-111$ 340.00BCL-10（B细胞淋巴瘤10）RP-112$ 290.00RAD51重组酶RP-113$ 220.00FGF-1（酸性成纤维细胞生长因子）RP-114$ 260.00FGF2成纤维细胞生长因子2（基本），（低分子量，134-278）RP-115$ 260.00SV40 T抗原（猿猴病毒40大抗原）RP-116$ 260.00FRA-1，类FOS抗原1RP-117$ 260.00MDM2（小鼠双分钟2）人类癌蛋白RP-118$ 260.00HIF-1a CTAD（缺氧诱导因子1 alpha C末端激活域，[776-826]）RP-119$ 250.00HIF-1a NTAD（缺氧诱导因子1 alpha N端激活域（530-698）RP-120$ 250.00MAD（MAX二聚化蛋白1，MXD1）RP-121$ 280.00MAX（Myc相关因子X）RP-122$ 260.00类核因子2，NRF2，NFE2L2，核因子类红细胞2相关因子2RP-123$ 250.00TNF-α（低内毒素）RP-124$ 220.00