ProductName	c-fos(protooncogene)
Size	5,000U
Description	ThetranscriptionfactorAP-1(activatorprotein-1)isinvolvedincellularproliferation,transformationanddeath.AP-1andnuclearfactorB(NFkB)canbespecificallytargetedtopreventcancerinductioninmousemodels.AP-1canbeproducedby18differentdimericcombinationsofproteinsfromtheJun[c-Jun(cat#P2014),JunBandJunD]andFos(c-Fos,FosB,Fra-1andFra-2)families,includingJunhomodimersandJun–Fosheterodimers(1–4).TheJunandFosproteinscontainabasic-regionleucinezipper(bZIP)domain,andarecapableofbindingtootherbZIPproteinsincludingthosefromtheATF,MAF,CNCandC/EBP(CCAAT/enhancer-bindingprotein)subfamilies(5).Jun–JunandJun–Fosdimersbindwithhighestaffinitytothe12-O-tetradecanoylphorbol-13-acetate(TPA)responseelement(TRE)[TGA(C/G)TCA](6),althoughmanyotherAP-1-likesiteshavebeenreported.Bindingtoanyofthesesitescanbetissue-specific,oraffectedbyneighboringsequences,anddependentuponinteractionswithothertranscriptionfactorsorcofactors.JunandFosproteinscanalsodimerizewithotherbZIPproteins,allowingthemtotargetotherDNAbindingsites,suchasthecAMPresponseelement(CRE),theantioxidantresponseelements(ARE),andhalf-sitescomposedofhalfofaTREsiteandhalfofaMAF-orCNC-bindingsite.Inaddition,AP-1proteinscaninteractwithotherproteins,includingthep65subunitofNF-B,CBP(CRE-binding-protein-bindingprotein)(p300),SMAD-3and-4,andtheretinoblastomaprotein(see5foramorecompletelist),furtherincreasingthecombinatorialpotentialofJunandFosproteins.AP-1regulatesavarietyofcellularprocesses,includingproliferation,differentiationandapoptosis,andcontributestobothbasalandstimulus-activatedgeneexpression.Itisactivatedbygrowthfactors,hormones,stress,cytokines,ROSandultravioletrADIation.Activationoccursbothtranscriptionallyandpost-translationally,andissignaledpredominantlythroughthemitogen-activatedproteinkinase(MAPK)cascade(7).ThecombinatorialdiversityofAP-1proteinsandotherinteractingfactorsappearstoinfluencehowspecificcelltypesrespondtoastimulus.
Applications	protein-DNAandprotein-proteininteractionassays
Source	Baculovirus-insectcell
FusionTag	6XHis
PurificationMethod	FPLC
UnitDefinition(Activity)	1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay.
Purity	~95%asdeterminedbySDS-PAGE
GeneID	6722
Accession#	NM_003131
GeneName	serumresponsefactor(c-fosserumresponseelement-bindingtranscriptionfactor)
GeneSymbol	SRF
GeneSynonyms	MCM1
MW	53.3kDa
ProteinSequence	MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREA AAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAA TGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTG TQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATG FEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPIT NYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAI QVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPT SGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAV IGQQAGSSSNLTELQVVNLDTAHSTKSE
StorageBuffer	20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage	-20to-80°C.
Shipping	4°Cordryice
References	1.CurranT.andFranzaB.R.Jr(1988)Cell55,395-397 2.RauscherF.J.D.etal.(1988)Science,240,1010-1016 3.RauscherF.J.D.etal.(1988)Cell52,471-480 4.WagnerE.F.(2001)Oncogene20,2333-2497 5.ChinenovY.andKerppolaT.K.(2001)Oncogene20,2438-2452 6.AngelP.etal.(1987)Cell49,729-739 7.RobinsonM.J.etal.(1998)Curr.Biol.8,1141-1150

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