Description
CatalogueNumber	16-246
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-EGFRAntibody,neutralizing,cloneLA1,AlexaFluor®488
Overview	IncludedNegativeControl:Catalog#16-240,AlexaFluor®488-conjugatedNormalMouseIgG. InhibitsA431cellgrowth.Recognizesanantigenicdeterminantlocatedontheextracellulardomainofthereceptor.

ProductInformation
Format	AlexaFluor®488

StorageandShippingInformation
StorageConditions	1yearat4°Cfromdateofshipment

Applications
Application	ThisAnti-EGFRAntibody,neutralizing,cloneLA1,AlexaFluor488isvalidatedforuseinIFforthedetectionofEGFR.
KeyApplications	Immunofluorescence

BIOLOGicalInformation
Immunogen	HumanA431membraneproteins
Clone	cloneLA1
Host	Mouse
Specificity	externaldomainofEGFreceptoronallhumancells.CompeteswithEGFandTGF-αforbindingonhumancells.Recognizesbothphosphorylatedandnon-phosphorylatedhumanEGFreceptors.
Isotype	IgG1
SpeciesReactivity	Human
SpeciesReactivityNote	DoesnotreactwithEGFreceptorsonmouseorratcells.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_005228.3 NM_201282.1 NM_201283.1 NM_201284.1
GeneSymbol	EGFR ERBB1 ERBB mENA
UniProtNumber	P00533
UniProtSummary	FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010