Description
CatalogueNumber	05-1050X
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-PhosphotyrosineAntibody,4G10®Platinum
BackgroundInformation	Thedevelopmentoftheanti-phosphotyrosine,clone4G10®in1989wasamonumentaldiscoveryforresearchers.4G10®wasthefirstandisthebestsinglemonoclonalantibodyforthedetectiontyrosinephosphorylation.4G10®iswellknownforitssensitivityanditsABIlitytodetectmultipletyrosinephosphorylationsonnumeroussubstrates.Ithasbeenvalidatedbythousandsofscientificandmedicalresearchersinvirtuallyeveryapplicationandtyrosinetargetoverthepast2decades.Toimproveonsomethingthathundredhavetriedandnoonehassucceeded,wepooled4G10®withthenextmosthighlyregardedanti-phosphotyrosine,clonePY20tomake4G10®Platinum.PY20itselfisaverypoorsubstitutefor4G10®,butitsadditiveeffectallowforagreaterlevelofdetectiononmoresubstratesthateven4G10®alonewasnotcapableof.

ProductInformation
Format	Purified
Control	SuggestedPositiveAntigenControl:Cat.#12-302,EGF-stimulatedA431celllysateisprovidedasafreepositiveantigencontrolforwesternimmunoblotting.Aliquotasdesired,refreezeimmediately,andstoreat–20ºC.Thelysateisstablefor6monthsat–20ºC.
Presentation	AproprietarymixtureofproteinGpurifiedmousemonoclonals4G10®(IgG2bκ)andPY20(IgG2b)in0.1MTris-glycine,pH7.4,0.15MNaCl,0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	2yearsat2-8°Cfromdateofshipment. NOTE:DONOTFREEZE. Formaximumrecoveryoftheproduct,centrifugetheoriginalvialpriortoremovingthecap.Iftheproducthasaccidentallybeenfrozenandthawed,spinitat13,000xgfor10minutesat2-8°C.Savethesupernatantforapplication.

Applications
Application	Anti-PhosphotyrosineAntibody,4G10PlatinumisanantibodyagainstPhosphotyrosineforuseinWB,IF,FC,IH,IP,IC,ELISA.
KeyApplications	WesternBlotting Immunofluorescence FlowCytometry Immunohistochemistry Immunoprecipitation Immunocytochemistry ELISA

BIOLOGicalInformation
Immunogen	4G10®usedPhosphotyraminecoupledtoKLHandPY20usedphosphotyrosineconjugatedtocarrierprotein.
Clone	4G10
Host	Mouse
Specificity	Tyrosine-phosphorylatedproteinsfromallspecies
Isotype	IgG2b
SpeciesReactivity	All
AntibodyType	MonoclonalAntibody
Modifications	Phosphorylation
PurificationMethod	ProteinG-Sepharose™Chromatography
MolecularWeight	Dependentuponthemolecularweightofthetyrosinephosphorylatedproteinbeingdetected.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010