Description
CatalogueNumber	APT116
BrandFamily	Chemicon®
TradeName	Apo-BrdU Chemicon
Description	Apo-BRDUIHCKit
Overview	TheCHEMICONAPO-BRDU-IHC™KitisatwocolorstainingmethodforlabelingDNAbreaksandtotalcellularDNAtodetectapoptoticcellsbyimmunohistochemistry(Lietal.1995).Thekitcontainstheinstructionsandreagentsrequiredformeasuringapoptosisincellsincluding:positiveandnegativecontrolslidesforassessingreagentperformance;reactionandblockingbuffersforprocessingindividualstepsintheassay;terminaldeoxynucleotidyltranferaseenzyme(TdT),bromodeoxyuridinetriphosphate(Br-dUTP),biotin-labeledanti-BrdUantibodyforlabelingDNAstrandbreaks,HRP-streptavidinconjugate,DAN,H2O2/Ureatabletsforcolorgeneration,andmethylgreensolutionforcounterstainingthecells.
MaterialsRequiredbutNotDelivered	1.Xylene-100% 2.Ethanol-100%,90%,80%,70%v/vwithdH2O 3.Methanol-100% 4.HydrogenPeroxide-30% 5.PBSorTris-bufferedsaline(1XTBS,20mMTrispH7.6,140mMNaCl) 6.10mMTris,pH8.0 7.1mMMgSO4in1XTBS(foruseingeneratingpositivecontrol) 8.DNaseI(foruseingeneratingpositivecontrol) 9.1-4%FormaldehydeinPBS,pH7.4 10.DistilledWater 11.Coplinjarswithslideholders 12.Beakerforrinsingslides 13.Slidehumidifyingchamber 14.Glasscoverslips 15.Mountingmedia 16.Microscope 17.Micro-pipettorwithdisposabletips 18.Microcentrifugetubes 19.Parafilm®(RegisteredtrademarkofAmericanNationalCanCompany) 20.Absorbentwipes 21.Crushedice

ProductInformation
Components	Positive/NegativeControlSlides,Naturalbox Note:2slides,eachcontainingamixturepositiveandnegativecontrolcells. BlockingBuffer,Whitecap H2O2/UreaTablets,Ambervial ProteinaseK,Pinkcap DABTablets,Ambervial TdTEnzyme,Yellowcap Br-Dutp,Violetcap 200XStreptavidin-HRPConjugate,Blackcap 5XReactionBuffer,Greencap Biotin-labeledBrdUmAb,Orangecap MethylGreensolution,Naturalcap, TheAPO-BRDU-IHC™Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival. Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed. Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
Detectionmethod	Chromogenic

StorageandShippingInformation

StorageConditions

PrecautionsandWarnings: 1.ThecomponentsofthiskitareforResearchUseonlyandarenotintendedfordiagnosticprocedures. 2.Afterinitialdefrosting,storethe5XReactionBufferandBiotin-labeledanti-BrdUantibodyat2-8ºC,andtheMethylGreensolutionatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed. 3.TdTEnzymewillnotfreezeat-20oC,becauseitisin50%(v/v)glycerolsolution. 4.The5XReactionbufferandBr-dUTPsolutioncontaincacodylicacidasabuffer.TheBiotin-labeledantibodycontains0.05%sodiumazideasapreservative.TheDABtabletscontaindiaminobenzidine.Thesematerialsareharmfulifswallowed.Avoidskincontactwithanyofthesecomponents;washimmediatelywithwater. 5.Uponwarmingthecomponents,centrifugethetubesfor30secondstoforcealltheliquidtothebottomofthetube. 6.Gloves,labcoatandprotectiveeyewearshouldbewornwhilestaining.

Applications
Application	TheAPO-BRDU-IHCKitisatwocolorstainingmethodforlabelingDNAbreaks&totalcellularDNAtodetectapoptoticcellsbyIHC.
KeyApplications	Immunohistochemistry

BIOLOGicalInformation
SpeciesReactivity	All

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010