Description
CatalogueNumber	M-004142
BrandFamily	Upstate
TradeName	Smartpool Upstate
Description	RasSMARTpool®siRNAreagent
Overview	ForquestionsorconcernsregardingtheuseoftheSMARTpools®,pleasecontactDharmaconTechnicalSupport,1-800-235-9880,email:lab@dharmacon.com.

ProductInformation

StorageandShippingInformation

Applications
KeyApplications	RNAi/siRNA/GeneKnockdown
ApplicationNotes	SMARTpools®arethecollectionofatleastfourindividualsiRNAduplexestargetedagainstaspecificgeneanddesignedusingabioinformaticstechnologyknownasSMARTselection™,resultinginthegenerationofsiRNAsthatfunctionmorethan97%ofthetime.Thetargetedmessagelevelisreducedbymorethan70%24hoursaftertransfection.Foruseinhumancelllines.

BIOLOGicalInformation
SpeciesReactivity	Human
EntrezGeneNumber	NM_001085049.1 NM_012219.3
EntrezGeneSummary	MembersoftheRASsuperfamilyofGTP-bindingproteins,whichincludesMRAS,aremembrane-anchored,intracellularsignaltransducersresponsIBLeforavarietyofnormalcellularfunctions.Theyareoncogenicallyactivatedinasignificantfractionoftumors.[suppliedbyOMIM]
GeneSymbol	MRAS RRAS3 M-RAS FLJ42964 R-RAS3 M-RAs
UniProtNumber	O14807
UniProtSummary	FUNCTION:SwissProt:O14807#Mayserveasanimportantsignaltransducerforanovelupstreamstimuliincontrollingcellproliferation.WeaklyactivatestheMAPkinasepathway. SIZE:208aminoacids;23846Da SUBUNIT:InteractswithRGL3(Bysimilarity). SUBCELLULARLOCATION:Cellmembrane;Lipid-anchor;Cytoplasmicside(Potential). TISSUESPECIFICITY:Expressionhighlyrestrictedtothebrainandheart. SIMILARITY:SwissProt:O14807##BelongstothesmallGTPasesuperfamily.Rasfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010