Description
CatalogueNumber	AB1772
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-SEROtoninTransporterAntibody

ProductInformation
Format	Serum
Presentation	Liquid,nopreservatives.

StorageandShippingInformation
StorageConditions	Maintainat-20°Cinundilutedaliquotsforuptooneyear.Avoidrepeatedfreeze/thawcycles.

Applications
Application	ThisAnti-SerotoninTransporterAntibodyisvalidatedforuseinIHforthedetectionofSerotoninTransporter.
KeyApplications	Immunohistochemistry
ApplicationNotes	Immunohistochemistry:1:5,000usingaCy3conjugatedsecondaryantibody.Thetissuewasfixedinaparaformaldehyde/picricacid-mixture(4%paraformaldehydeand0.4%picricacidin0.16Msodiumphosphatebuffer,pH6.9).Seeapplicationnotes. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	Syntheticpeptidefromthecarboxy-terminusoftheclonedratserotonintransporter.
Host	GuineaPig
Specificity	SerotoninTransporter.Theantiserumhasbeentestedontissuesectionsfromthecentralnervoussystem.ThestainingpatternobtainedwiththeAB1772correspondstothepatterndescribedusingantiserumtoserotoninitself.Theserotonintransporterimmunoreactivitycorrespondstowhathasbeendescribedusingotherantibodiestotheserotonintransporter.Preabsorptionoftheantiserumwiththeimmunogenpeptidecompletelyabolishestheimmunostaining.
SpeciesReactivity	Rat
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_001045.3
EntrezGeneSummary	Thisgeneencodesanintegralmembraneproteinthattransportstheneurotransmitterserotoninfromsynapticspacesintopresynapticneurons.Theencodedproteinterminatestheactionofserotoninandrecyclesitinasodium-dependentmanner.Thisproteinisatargetofpsychomotorstimulants,suchasamphetaminesandcocaine,andisamemberofthesodium:neurotransmittersymporterfamily.Arepeatlengthpolymorphisminthepromoterofthisgenehasbeenshowntoaffecttherateofserotoninuptakeandmayplayaroleinsuddeninfantdeathsyndrome,aggressivebehaviorinAlzheimerdiseasepatients,anddepression-susceptibilityinpeopleexperiencingemotionaltrauma.
GeneSymbol	SLC6A4 5-HTT 5HTT HTT OCD1 hSERT SERT
UniProtNumber	P31645
UniProtSummary	FUNCTION:SwissProt:P31645#Terminatestheactionofserotoninbyitshighaffinitysodium-dependentreuptakeintopresynapticterminals. SIZE:630aminoacids;70325Da SUBUNIT:InteractswithTGFB1I1. SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. SIMILARITY:SwissProt:P31645##Belongstothesodium:neurotransmittersymporter(SNF)family. MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010