Description
CatalogueNumber	AB15417
Replaces	MABN16
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-Beclin1Antibody
AlternateNames	Coiled-coilmyosin-likeBCL2-interactingprotein BECN1
BackgroundInformation	Autophagyistheprocessbywhichcellsrecyclecytoplasmanddisposeofexcessordefectiveorganelles.Thisprocessissuggestedtobeinvolveddevelopment,differentiation,growthregulationandtissueremodelinginmulticellularorganisms.Beclin1isaBCL-2interactingproteinandthisproteinwasshowntoplayaroleinthepromotionofautophagy.

ProductInformation
Format	Serum
Presentation	Lyophilized.Reconstitutewith50μLofsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.Containsnopreservative.

StorageandShippingInformation
StorageConditions	Maintainlyophilizedmaterialfrozenat-20°Cto-70°Cforupto12monthsafterdateofreceipt.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.Glycerol(1:1,ACSorbettergrade)canbeaddedforadditionalstABIlity.

Applications
Application	DetectBeclin1usingthisAnti-Beclin1AntibodyvalidatedforuseinWB,IC,IH.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Westernblot:1:100-1:2,000 Immunocytochemistry:1:100-1:2,000 Immunohistochemistry:1:100-1:2,000 Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	SyntheticpeptidecorrespondingtotheC-terminusofhumanBeclin1protein.
Host	Rabbit
Specificity	Beclin1[Coiled-coilmyosin-likeBCL2-interactingprotein,BECN1].
SpeciesReactivity	Human
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_003766.2
GeneSymbol	BECN1 GT197 Beclin-1 beclin1 ATG6 VPS30
PurificationMethod	Serum
UniProtNumber	Q14457
UniProtSummary	FUNCTION:SwissProt:Q14457#Mayplayaroleinantiviralhostdefense.ProtectsagainstinfectionbyaneurovirulentstrainofSindbisvirus.Mayplayaroleinautophagy. SIZE:450aminoacids;51896Da SUBUNIT:InteractswithGOPCandGRID2(Bysimilarity).InteractswithBCL2andBCL2L1. SUBCELLULARLOCATION:Cytoplasm(Potential).Golgiapparatusmembrane;Peripheralmembraneprotein(Bysimilarity).Note=ExpressedindendritesandcellbodiesofcerebellarPurkinjecells(Bysimilarity). TISSUESPECIFICITY:Ubiquitous. SIMILARITY:SwissProt:Q14457##Belongstothebeclinfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010