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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Alternative Name MT3, MT-3
Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Bioassay Tested Bioassay Tested
Every lot is tried & tested in a relevant biological assay.
Our Bioassay
Origin Natural protein isolated from Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
MW: 7379 Da.
Purity: >99% (HPLC)
Form Lyophilized powder.
Effective concentration 100-1000 nM.
Sequence LTCVTKNTIFGITTENCPAGQNLCFKRWHYVIPRYTEITRGCAATCPIPENYDSIHCCKTDKCNE.
Modifications Disulfide bonds between Cys3-Cys24, Cys17-Cys42, Cys46-Cys57and Cys58-Cys63.
Molecular formula C319H489N89O97S8.
Activity Muscarinic receptor 4 and adrenoceptor antagonist1-4.
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- Jolkkonen, M. et al. (1994) FEBS Lett. 352, 91.
- Karlsson, E. et al. (1994) Ann. N.Y. Acad. Sci. 710, 153.
- Caulfield, M.P. and Birdsall, N.J. (1998) Pharm. Rev. 50, 279.
- Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
Accession number P81031.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
-
Alomone Labs Muscarinic Toxin 3 inhibits Muscarinic Receptor 4 expressed in CHO-K1 cells.CHO-K1 cells were co-transfected with human M4 and Gα15 in order to couple the M4 receptor to the Ca2+ signaling pathway. Ca2+ concentrations were monitored using FLIPR. Cells were first incubated in the presence of different concentrations of Muscarinic Toxin 3 (#M-140) followed by Oxotremorine. The percentage of M4 receptor inhibition was plotted versus different concentrations of Muscarinic Toxin 3. The IC50 value of Muscarinic Toxin 3 was between 100 to 1000 nM.
References - Scientific background
- 1. Bradley, K.N. (2000) Pharmacol. Ther. 85, 87.
- 2. Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
- Muscarinic toxins are isolated from the venom of the mamba species and are composed of 65 to 66 amino acid residues with four intramolecular disulfide bridges. One of these is muscarinic toxin 3 (MT-3) which was isolated from the green mamba (Dendroaspis augusticeps) and is composed of 65 amino acid residues1. A recent study investigated the interaction of MT3 with cloned receptors (mAChRs and adrenoceptors) expressed in insect cells by radioligand binding. MT3 appears to have a broad spectrum of targets showing high-affinity binding (IC50 = 1-10 nM) to M4 mAChR, α1A-, α1D- and α2A-adrenoceptors and lower affinity binding (IC50 > 25 nM) to α1B- and α2C-adrenoceptors and M1 mAChR2.
Target M1, M4 muscarinic receptors, adrenoceptors
Net Peptide Content: 100%
Lyophilized Powder
Click here to receive a 50 µg free trial sample!
Last update: 04/11/2019
Muscarinic Toxin 3 (#M-140) is a highly pure, natural, and biologically active peptide toxin.
For research purposes only, not for human use
Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司，也是抗体研究市场的先驱，目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体（早期药物“发现”），并将补充我们非常成功且长期存在的定制抗体业务（www.crbdiscovery.com）。我们已经以新颖，复杂，稀有和翻译后修饰的特异性抗体而闻名，这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。 我们于2017年2月，在发现肽之后一年，并且在詹姆斯·库克船长（他当时最伟大的探险家）于1776年从英国出发，乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此，“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间，还有其他相似之处。 有关更多详细信息，请参见有关Discovery®抗体发布的新闻稿。 Cambridge Research Biochemicals （www.crbdiscovery.com）成立于1980年，是英国领先的定制肽和抗体工具的独立生产商，为全球制药，生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂，用于早期药物发现中的主要研究证据，并支持临床研究中的生物标志物计划。该公司生产各种口味的肽，从简单到修饰，稳定和放射性标记，再到从毫克到克到最高纯度的染料标记。此外，CRB是产生高度特异性和靶向抗体（多克隆抗体和单克隆抗体）的公认专家。荧光染料标记也是重点。自1994年以来，该公司于2016年启动了其首个目录业务DISCOVERY®。
