发现抗体/抗降钙素受体(细胞外)抗体/25µl/ACR-053-25µl

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¥4940.00
货号:ACR-053-25µl
浏览量:127
品牌:Discovery
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商品描述
Cat#:ACR-053
AlternativeNameCALCR,CTreceptor,CT-R,C1A/C1B
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:m,r
    Mayalsoworkin:h*
      • Peptide(C)HLVEVVPNGDLVRRD,correspondingtoaminoacidresidues201-215ofratCALCR(Accession P32214).
        Anti-Calcitonin Receptor (extracellular) Antibody
    Accession(Uniprot)NumberP32214
    GeneID116506
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse–identical;human–14/15aminoacidresiduesidentical.
    RRIDAB_2756577.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.85mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,lci,wb
    Mayalsoworkin:ifc*,ih*,ip*
      • Anti-Calcitonin Receptor (extracellular) Antibody
        Westernblotanalysisofratbrainmembrane(lanes1and3)andmousebrainlysate(lanes2and4):
        1,2. Anti-CalcitoninReceptor(extracellular) Antibody(#ACR-053),(1:200).
        3,4.Anti-CalcitoninReceptor(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-Calcitonin Receptor (extracellular) Antibody
        ExpressionofCALCRinratPC12cells
        Cellsurfacedetectionof CALCRinintactlivingratpheochromocytoma(PC12)cells.A,C.Extracellularstainingofcellsusing Anti-CalcitoninReceptor(extracellular) Antibody,(#ACR-053),(1:50).B,D.MergedimagesofAandC,eachwithitsrespectiveliveviewofthecells.
    References
    • 1.Segovia-Silvestre,T.etal.(2011)BMCRes.Notes4,407.
    • 2.Barwell,J.etal.(2012)Br.J.Pharmacol.166,51.
    • 3.Taboulet,J.etal.(1998)Hum.Mol.Genet.7,2129.
    • 4.Gorn,A.H.etal.(1995)J.Clin.Invest.95,2680.
    • 5.Gorn,A.H.etal.(1992)J.Clin.Invest.90,1726.
    • 6.Gooi,J.H.etal.(2014)J.Endocrinol.221,181.
      • thecalcitoninreceptor(CTR)isaclassB G-proteincoupledreceptor (GPCR)thatsignalsviaactivationofcAMPandproteinkinaseC(PKC).Itisoneof15humanclassBGPCRs1,2.ApartfromCTR,calcitoninreceptor-likereceptor(CTLR)istheonlyotherB-classGPCRtobindcalcitonin.Todate,therearethreeknownhumanCTRmRNAisoformsarisingfromalternativesplicing3.

        Calcitoninitselfisapeptidehormonethatcontributestobonecalcificationbydirectinhibitionofosteoclastswhichresorbthebonytissue.Itispartofasix-memberfamilythathasvariedfunctionalities.Bybindingtoreceptoractivitymodifyingproteins(RAMPs),CTRandCTLRcanformreceptorstoeverymemberofthecalcitoninfamily.Forinstance,RAMP1andCTRconstructthecalcitoningene-relatedpeptide(CGRP)receptor.

        BothCTRandCTLRhaveseventransmembranedomains,anintracellularC-terminal,andanextracellularN-terminal.Toactivatethereceptors,theirN-terminalmustfirstcapturetheC-terminaloftheligandandthencarriedtothetransmembraneloopswhereitbestowsaconformationalchangeuponthem.

        Besidesthebone,CTRhasprominentrolesinthekidney,CNS,placenta,ovaries,sperm,lung,andinthebrain4,5.
        Inosteoclasts,theexpressionofCTRsdeclineswithage.Interestingly,polymorphismoftheCTRgeneisstronglyassociatedwithdecreasedfracture-riskinpost-menopausalwomen3,6.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:10/11/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofratCT-R.Anti-CalcitoninReceptorAntibody(#ACR-053)canbeusedinwesternblotandlivecellimagingapplications.IthasbeendesignedtorecognizeCT-Rfromhuman,ratandmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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