• Species Reactivity
  Mouse
• Specificity
  Detects mouse 4-1BB in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLymphotoxinbeta R, rmNGF R, rmOPG, rmOX40, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM.
• Source
  Monoclonal Rat IgG_2B Clone # 158306
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
  Val24-Leu187
  Accession # P20334
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B)
  under non-reducing conditions only
• Blockade of Receptor-ligand Interaction
  In a functional ELISA, 0.3 to 1.5 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of biotinylated Recombinant Mouse 4-1BB Ligand to immobilized Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera (Catalog # 937-4B) coated at 1 µg/mL (100 μL/well). At 20 µg/mL, this antibody will block >90% of the binding.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Kwon, B.S. and S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963.
  4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262.
  5. Kwon, B., H.W. Lee and B.S. Kwon, 2002, TRENDS in Immunology 23:378.
• Entrez Gene IDs:
  3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
• Alternate Names:
  4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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