- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Description: Purified Bovine Collagen Type I (Atelocollagen) from bovine tendons
Form: Lyophilized, salt free, 30 mg/ vial
Sterility: Passed sterility test for bacteria and fungi
Source: Bovine tendons
Purity: Bovine Collagen Type I (Atelocollagen) > 95%
Purification: partial pepsin digestion in acidic conditions and differential salt precipitation.
Reconstitution: Dissolve in 10-50 mM acetic acid. Suspension should be gently stirred for several hours or overnight at 4°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml.
Storage: Bovine Collagen Type I (Atelocollagen) dissolved in acetic acid is stable at 4°C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Application: Coating material for cell culture studies or 3D gel formation.
Literature/Support
Bovine Collagen Type 1 (Atelocollagen) Insert (PDF)
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Dissolve in 10-50 mM acetic acid. Suspension should be gently stirred for several hours or overnight at 4°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml.
