Anti-IKKβ, total Antibody

IκB kinases (Ikks) are related kinases that play a major role in the regulation of NF-kB (nuclear factor kB) activation, a transcription factor activated in response to infectious agents and pro-inflammatory cytokines. NF-kB is sequestered in the cytoplasm in an inactive form by the IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB, thereby preventing translocation of NF-kB to the nucleus. Activation of NF-κB is mediated by phosphorylation of IκB on specific serine residues, which results in targeted proteasome-mediated degradation of IκB. This releases NF-kB dimers from the cytoplasmic NF-kB - IkB complex allowing NF-kB to translocate to the nucleus where it regulates the transcription of numerous target genes. The serine phosphorylation of IkB is regulated by the IKK complex. The IKK complex is comprised of at least three tightly associated subunits: IKKα, IKKβ and IKKγ. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit.