TheViABIlity/CytotoxicityAssayKitforAnimalLive&DeadCellsprovidesgreen/redfluorescentstainingofliveanddeadcells,respectively.Theassayemploystwoprobesthatdetectintracellularesteraseactivityinlivecellsandcompromisedplasmamembraneintegrityindeadcells.

TheViability/CytotoxicityAssayKitforAnimalLive&DeadCellsprovidesgreen/redfluorescentstainingofliveanddeadcells,respectively.Theassayemploystwoprobesthatdetectintracellularesteraseactivityinlivecellsandcompromisedplasmamembraneintegrityindeadcells.TheesterasesubstratecalceinAMstainslivecellsgreen,whilethemembrane-impermeableDNAdyeEthidiumhomodimerIII(EthD-III)stainsdeadcellsred.Thekitissuitablefordetectionusingfluorescencemicroscopy,fluorescencemicroplatereader,orflowcytometry.Theassayprinciplesaregeneralandapplicabletomosteukaryoticcelltypes,includingadherentcellsandcertaintissues,butnottobacteriaoryeast.Thisfluorescence-basedmethodofassessingcellviabilitycanbeusedinplaceoftrypanblueexclusion,⁵¹Crrelease,andsimilarmethodsfordeterminingcellviabilityandcytotoxicity.EthD-III(#40050and#40051)isaproprietaryDNAstaindevelopedbyBiotiumtobeasuperioralternativetothespectrallysimilardyeEthidiumhomodimerI(EthD-I).EthD-IIIhashigherDNAbindingaffinityandthusbrighterfluorescence(30%brighterthanEthD-I).

SpectralProperties
Calcein(endproductafterhydrolysisofcalceinAM):
Ex/Em:494/517nm(pH8)

EthidiumhomodimerIII(EthDIII):
Ex/Em:530/620nm(withDNA)

Biotium 5(6)-羧基-X-罗丹明-N-羟基琥珀酰亚胺酯 Biotium 异硫氰酸荧光素酯 Biotium 10-壬基吖啶橙溴化物 Biotium 4-甲基伞形酮磷酸二钠盐三水 Biotium 河豚毒素 Biotium 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐 Biotium N,N-二甲基甲酰胺 Biotium N-生物素酰基-L-赖氨酸酰肼 Biotium 5-溴-6-氯-3-吲哚基-beta-D-半乳糖苷 Biotium N,N,N',N'-四(2-吡啶甲基)乙二胺 Biotium 腔肠素-H Biotium Α-金环蛇毒 Biotium 5-溴-4-氯-3-吲哚半乳糖苷 Biotium BIOTIN-(AC5)2-OSU Biotium 4',6-二脒基-2-苯基吲哚二盐酸盐 Biotium 4-甲基伞形酮磷酸酯 Biotium 90% (TLC) Biotium 96314-98-6 SULFORHODAMINE G 5873-16-5 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 127770-45-0 HYDROETHIDINE 38483-26-0 SULFORHODAMINE 101 60311-02-6 L-N6-(1-IMINOETHYL)LYSINE DIHYDROCHLORIDE 150403-89-7