DescriptionViPrimePLUS Taq qPCR Green Master Mix II is next generation master mix designed for fast and easy realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains pure Taq DNA Polymerases, EvaGreen® dye, highest quality dNTPs, and buffer components at optimal concentrations. Taq DNA Polymerases in the master mix provide excellent results in reaction efficiency, correlation coefficient and slope. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS Taq qPCR Green Master Mix II can be used to amplify any DNA template including genomic, cDNA and viral sequences. The formulation of qPCR green master mix can detect low copy number targets very specifically with high efficiency. The qPCR green master mix provides convenient and robust set up for quantitative real-time analysis of DNA samples.ViPrimePLUS Taq qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS Taq qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.

Applications

Features

• Ready-to-use real-time PCR reaction set up
• Rapid extension rate for early Ct values
• Good buffer system for excellent amplification efficiency
• Contain EvaGreen® dye – highest dye stability and safety
• High sensitivity detection
• Minimal PCR inhibition
• Compatible with most of the real-time PCR platforms

Component1.6ml aliquots of master mix

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Product Description	Compatible Hardware
QLMM17ViPrimePLUS Taq qPCR Green Master Mix II (EvaGreen® Dye)	Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™
QLMM17-LRViPrimePLUS Taq qPCR Green Master Mix II with Low ROX (EvaGreen® Dye)	Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7
QLMM17-RViPrimePLUS Taq qPCR Green Master Mix II with ROX (EvaGreen® Dye)	Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS

Ordering Information

Catalog No	Description	Pack Size
QLMM17	ViPrimePLUS Taq qPCR Green MasterMix II (EvaGreen® Dye)	150 reactions
QLMM17-LR	ViPrimePLUS Taq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye)	150 reactions
QLMM17-R	ViPrimePLUS Taq qPCR Green MasterMix II with ROX (EvaGreen® Dye)	150 reactions

DownloadManual

ViPrimePLUS Taq qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS Taq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS Taq qPCR Green MasterMix II with ROX (EvaGreen® Dye)

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