Recombinant Human CUL1/RBX1 Complex Protein, CF Summary

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CUL1/RBX1 Complex

Cullin-1 (CUL1) is a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, CUL1 serves as a scaffold that organizes the SKP1-F-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. This complex consists of an N-terminal 10-His tagged CUL1 and an untagged RBX1.

Related Research Areas

• Cell Cycle
• Deubiquitinating Enzymes (DUBs)
• Search Ubiquitin-related Enzymes
• Toll-Like Receptors