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TheCARReceptorBoosterrenderscellsthatcannotusuallybetransducedwithadenovirussusceptIBLetoinfection.Itcanalsobeusedtoincreaseadenoviraltransductionefficiencyinallcelltypes.Thereceptor-coatedcell-derivednanovesiclescombinewiththeplasmamembraneofyourtargetcellstotemporarilyelevatethedensityoftheadenovirusreceptorproteinCARonthecellsurface.
TheCARReceptorBoosterrenderscellsthatcannotusuallybetransducedwithadenovirussusceptibletoinfection.Itcanalsobeusedtoincreaseadenoviraltransductionefficiencyinallcelltypes.Thereceptor-coatedcell-derivednanovesiclescombinewiththeplasmamembraneofyourtargetcellstotemporarilyelevatethedensityoftheadenovirusreceptorproteinCARonthecellsurface.Forexample,hematopoieticcellsorCHOcells,whichareusuallyresistanttoadenoviralinfection,canbetransducedfollowingasimplepretreatmentwithCARReceptorBooster(seedataimagesbyexpandingtheDetailsarrowintheproducttablebelow).
Howdoesitwork?
TheCARReceptorBoosterconsistsofavialofconcentratedcell-derivednanovesiclesthataredenselycoatedwiththecoxsackievirusandadenovirusreceptorprotein(CAR).Whentheboostervesiclesareappliedtoyourcells(10–20µlperwellofa6-wellplatefor~2hr),theyfusewiththeplasmamembrane,resultinginanincreasedlevelofthereceptoronthecellsurface.Yourtargetcellscanthenbemoreefficientlytransducedwithrecombinantadenovirus.


