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Key Features
- Optimized to work with all Full Moon BioSystems’ antibody microarrays and maximize protein yield, labeling efficiency
- Provides major reagents required for lysate preparation, protein labeling, coupling and detection
- Efficient, reliable and easy to use
- This is a 2-reaction Antibody Array Assay Kit for processing two antibody array slides.
- Additional size available: 20-reaction kit for processing 20 slides
Kit Components
Biotin Reagent
Blocking Reagent
Coupling Chamber
Coupling Reagent
Detection Buffer
Extraction Buffer
Dry Milk Powder
Labeling Buffer
Spin Columns
Stop Reagent
10X Wash Buffer
Product Details
User’s Guide: Antibody Array User’s Guide
Size: 2 Reactions
Storage Condition: 4°C
Shelf-life: One year
Material Safety Data Sheet (MSDS)
Additional Reagent Needed
Streptavidin Conjugate — Cy3-Streptavidin is commonly used. It can be purchased from any vendors including GE Healthcare (PA43001), Invitrogen (S-32355, Alexa Fluor 555 streptavidin), Sigma (S6402) and others. Alternative dye streptavidin conjugates may be used in place of Cy3-streptavidin if the dye is compatible with your array imaging system.
抗体芯片种类: 抗体芯片特点: 50uL的液质样本、200ug的总蛋白量即可完成多指标检测; 适用于血清/浆、培养上清及细胞、组织裂解液等多种生物样本; 可以有效避免采用传统方法所引入的批次间实验误差; 扩大单个样本的信息量并可做不同分析物间的交互分析。 抗体芯片原理: 抗体芯片文献: Izzotti A, et al. Relationships between pulmonary microRNA and proteome profiles, systemic cytogenetic damage and lung tumors in cigarette smoke-exposed mice treated with chemopreventive agents. Carcinogenesis, 2013, 34(10): 2322-2329. Straussman R, et al. Tumour microenvironment elicits innate resistance to RAF inhibitors through HGF secretion. Nature, 2012, 487(7408): 500-504. Bagnis A, et al. Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma. Exp Eye Res, 2012, 103: 55-62. Moon K M, et al. The effect of secretory factors of adipose-derived stem cells on human keratinocytes. Int J Mol Sci, 2012, 13(1): 1239-1257. Saccà S C, et al. New proteins as vascular biomarkers in primary open angle glaucomatous aqueous humor. Invest Ophth Vis Sci, 2012, 53(7): 4242-4253. Yalcin A, et al. Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclindependent kinases. J Bio Chem, 2009, 284(36): 24223-24232. Tang Y L, et al. Hypoxic preconditioning enhances the benefit of cardiac progenitor cell therapy for treatment of myocardial infarction by inducing CXCR4 expression. Circ Res, 2009, 104(10): 1209-1216.


