Tulip Biolabs蚂蚁淘在线

InhibitionofPARP1by3-aminobenzamide

AutomodificationofPARP1

SDS-PAGE/Coomassieblue

AutomodificationofPARP1WBusinganti-PARP1

●PARP1Enzymehuman, highlyactivePoly(ADP-ribose)polymerase-1(PARP1)isanabundantandubiquitousnuclearenzymethatcatalyzestheNAD(+)-dependentadditionofADP-ribosepolymersonavarietyofnuclearproteins. TheTulipBiolabs,Inc. PARP1Enzyme,Cat.#2090,ishighlypurifiedhumanPARP1,expressedinabaculovirusexpressionsystem.ThePARP1hasahighspecificactivity,approx.20000U/mg(1U=10fmolADP-riboseincorporatedintoimmobilizedhistonein30min@RT). Itislyophilizedfromitsnativestateunderspecialconditions,anduponreconstitutionwithwater,itretainsitspolyADP-ribosylationenzymaticactivity. PuritybySDS-PAGEis>95%. Eachvialcontains50µgPARP1(approx.1000Units).

●Useinhigh-throughputenzymeactivity/inhibitionassays

●Poly-ADP-ribosylationoftargetproteins

●WesternBlot,100ng/laneusingHRP/TMBcolorimetricmethods

●ELISA,2ng/well

●SDS-PAGE,1µg/lane●Asastandardinotherassays

Pleaseseethe Cat.#2090Datasheet(pdf) foracompletedescription.

专门设计并经过现场测试的96孔微孔板ELISA试剂盒，用于检测合成大麻素JWH-018，JWH-073，JWH-122，JWH-019，JWH-081，AM-2201和相关化合物以及几种它们在尿液中的代谢产物。已对该工具包的性能进行了分析，并将结果发表在同行评审的期刊上（请参阅 Arntson 等，2013 ）。https://academic.oup.com/jat/article/37/5/284/786497