Description

7501-500-k_calculation_worksheet

IntheHTSuperoxideDismutaseAssay,superoxiderADIcalions,generatedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertWST-1toWST-1-formazan.WST-1-formazanabsorbslightat450nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofWST-1formazanformation.TheextentofreductionintheappearanceofWST-1formazanisameasureofSODactivitypresentinexperimentalsamples.Thisassayisfreeofinterferencebyothercatalyticactivities,andunlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.

FEATURES:

• 96wellformatforhighthroughputscreening
• Assayperformedinaslittleas6minutes
• Suitableformammaliantissuesandcelllysates
• Suitablefortheassayof(Mn²⁺)-SOD,(Fe²⁺)-SOD,and(Cu/Zn)-SOD

APPLICATIONS:

CalculatingrelativeSODactivitybymeasuringthepercentinhibitionoftherateofformationofWST-1formazan.

 

Catalog#7501-500-Kincludes:
CatalogNumber	Description	Qty
7501-500-01	SODStandard	1
7501-500-03	XanthineOxidase(XOD)	1
7501-500-02	10XSODBuffer	1
7501-500-04	10XXanthineSolution	1
7501-500-05	20%TritonX-100	1
7501-500-06	WST-1Reagent	1
7512-100-06	96-WellPlate	1

 

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。