Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
ProteinA,similartoProteinG,isanimmunoglobulin-bindingproteinexpressedingroupCandGStreptococcalbacteriabutwithdifferentspecificities.ProteinAmaybeconjugatedwithvariousreportermoleculesincludingfluorescentdyes(FITC),enzymeMarkers(peroxidase,α-galactosidase,alkalinephosphatase),biotin,andcolloidalgoldwithoutaffectingtheantibodybindingsiteonthemolecule.Additionally,ProteinAmaybeimmobilizedonasolidsupportsuchasagaroseoracrylicbeadsforthepurificationofeitherpolyclonalormonoclonalimmunoglobulins.Itisalsoroutinelyusedforimmunoprecipitationassays.ProteinAisacellwallcomponentproducedbyseveralstrainsofStaphylococcusaureusthatconsistsofasinglepolypeptidechainandcontainslittleornocarbohydrate.TheProteinAmoleculecontainsfourαigh-affinity(Ka=108/mole)bindingsitescapableofinteractingwiththeFcregionfromIgGofseveralspeciesincludinghumanandrabbit.OptimalbindingoccursatpH8.2,althoughbindingalsoexistsbetweenpH7.0-7.6.

Specifications:

CatalogNo.	1001-01
CountryofManufacture	UnitedStates
ProductCode	rProteinA
Size/Quantity	5mg
ProductUse	Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Storage	Storeat-20°Cafterreceiving.UponReconstitution,storeat2-8°Cforuptooneweek.FormaximalstABIlity,aliquotandstoreat-20°C.Avoidrepeatedfreeze/thawcycles.
ShippingInfo	Dryice
Source	Escherichiacoli
MolecularWeight	Approximately45kDa,consistingof5IgGbindingdomainsE-D-A-B-Calignedinseries.A280of0.1%solution
Purity	0.165
BIOLOGicalActivity	>95%bySDS-PAGEanalyses.SpecificityTheinteractionbetweenProteinAandIgGisnotequivalentforallspecies.Evenwithinaspecies,ProteinAinteractswithsomesubclassesofIgGandnotothers.
PhysicalAppearance	Whitelyophilizedpowder.
Formulation	Lyophilizedwithnoadditives.
Endotoxin
Reconstitution	Reconstituteinsteriledistilledwaterorsaline.

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