Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
LeukemiaInhibitoryFactor(LIF)isalymphoidfactorwhichpromoteslong-termmaintenanceofembryonicstemcellsbysuppressingspontaneousdifferentiation.LIFhasanumberofotheractivitiesincludingcholinergicneurondifferentiation,controlofstemcellpluripotency,boneandfatmetabolism,mitogenesisofcertainfactordependentcelllinesandpromotionofmegakaryocyteproductioninvivo.

Specifications:

CatalogNo.	123-07
CountryofManufacture	UnitedStates
ProductCode	rMuLIF
Size/Quantity	5μg
ProductUse	Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Storage	Storeat-20°Cafterreceiving.Uponreconstitution,storeat2-8°Cforuptooneweek.FormaximalstABIlity,aliquotandstoreat-20°C.Avoidrepeatedfreeze/thawcycles.
ShippingInfo	Dryice
Synonyms	D-FACTOR,differentiation-stimulatingfactor
AASequence	MSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF
Source	Escherichiacoli
MolecularWeight	Approximately20kDa,asinglenon-glycosylatedpolypeptidechaincontaining181aminoacids.
Purity	>98%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivity	ActivitydeterminedbyitsabilitytoinducedifferentiationofmurineM1myeloidleukemiccells.Minimumdetectableconcentrationinassayis0.5ng/mL,correspondingtospecificactivity>1x
PhysicalAppearance	Whitelyophilizedpowder
Formulation	Lyophilizedfroma0.2μmfilteredconcentrated(1mg/ml)solutionin20mMPB,pH7.4,with0.02%TWEEN20.
Endotoxin
Reconstitution	Reconstituteinsteriledistilledwatercontaining0.1%BSAtoaconcentrationof0.1-1.0mg/mL.

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