IsolatedfromadultC57BL/6mousebonemarrow.Cellsareharvestedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.

Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.Inthebonemarrowandsubsequestlyinthebloodandtissues,monocytesundergoacascadeofmaturationthatculminateinmorphologicallyandfunctionallymaturetissuemacrophages[1].MacrophagesfunctiontoremovalcellulardebrisandinvADIngpathogens.Theyphagocytizeinvadingmicroorganisms,andscavengedead,damagedcellsandcellulardebris.MacrophagescanbeidentifiedbythespecificexpressionofseveralcellsurfaceproteinsincludingCD14,CD11b,F4/80(mice)/EMR1(human),MAC-1/MAC-3,andCD68byflowcytometryorimmunohistochemicalstaining[2].

MMa-bmfromSciencellResearchLaboratoriesareisolatedfromadultmousebonemarrow.MMa-bmareharvestedatP0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MMa-bmarecharacterizedbyimmunofluorescencewithantibodyspecifictoCD11bandF4/80. MMa-bmarenegativeformycoplasma,bacteria,yeast,andfungi.MMa-bmareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,MMa-bmarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.

RecommendedMedium

ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)fortheculturingofMMa-bminvitro.

Specifications:

CatalogNo.	M1920-57
CountryofManufacture	UnitedStates
ProductCode	MMa-bm
Size/Quantity	1x10^6cells/vial
ProductUse	MMa-bmareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage	Transfercellsdirectlyandimmediatelyfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments.
ShippingInfo	Dryice.
References	[1].ClineMJandSumnerMA.(1972)BoneMarrowMacrophagePrecursors.I.SomeFunctionalCharacteristicsoftheEarlyCellsoftheMouseMacrophageSeries.Blood.40:62-69.[2].WynnTAandBarronL.(2010)Macrophages:masterregulatorsofinflammationandfibrosis.SeminLiverDis.30:245-57.[3].GordonSandTaylorRP.(2005)Monocyteandmacrophageheterogeneity.NatureReviewsImmunology.5:953-964.

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。