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蚂蚁淘在线 / 品牌中心 / GenWay / GenWay/TMB核心+试剂/GWB-Q00248/100毫升

GenWay/TMB核心+试剂/GWB-Q00248/100毫升

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￥4040.00

货号：GWB-Q00248

浏览量：127

品牌：GenWay

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AdditionalInformation:

GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits

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/**/

Name	TMBCORE+Reagent
RelatedProductNames	TMBCORE+Reagents,ELISASolutions,TMBCORE+
ProductFormat	ReadytouseTMBsolution-liquid
Applications	ELISA
ApplicationInfo	ELISA:Neat
Format	ReadyToUse
StABIlity	48monthsfromdateofdespatch.
ReconstitutionandStorage	Storeat+4^oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethylene(HDPE). ShelfLife 48monthsfromdateofdespatch.
Storage	Storeat+4^oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethy
LeadTime	Domestic:within2-3weeksdelivery International:2-3weeks
IntendedUse	ResearchUseOnly
::	IntendedUse:ThisproductisahighperformanceTMB(3,3",5,5"-tetramethylbenzidine)solution,recommendedforuseinELISAasasubstrateforhorserADIshperoxidase(HRP). ThisproductcontainsTMB,substratebufferandhydrogenperoxideinasafe,readytousesolution.TheactivityofTMBhasbeenoptimisedtoenableincreasedsensitivity,minimalbackgroundandrapiddevelopment. ThisproductproducesadeepbluecolourduringtheenzymaticdegradationofH₂O₂byhorseradishPeroxidase.Thereactionmaybestoppedwith0.2Msulphuricacid,resultinginayellowcolourreadat450nm. TMBPLUS2hassimilarsensitivitytoTMBPLUS(GWB-Q00251orGWB-Q00252),butanincreasedshelflife.
::	InstructionsForUse: 1.Itisrecommendedthat100ulofGWB-Q00248TMBsubstrateisusedpermicrotiterwell.Pourthedesiredamountofsubstrateintoasealedcontainerandallowittoreachroomtermperatureinthedark. 2.Add100ulsubstratesolutionpermicrotiterwell. 3.Allowdevelopmentofthesubstratesolution.Timeofdevelopmentistypically5-30minutes.Forbestresults,theplateshouldbekeptinthedarkduringincubatione.g.wrappedintinfoil. 4.Forkineticassays,readabsorbanceat655nm(blue).Forendpointassays,addanequalvolumeof0.2Msulphuricacidandreadtheabsorbanceat450nm(yellow).Thisendpointsolutionisstableforuptoonehour. N.B.Ifreducedintensityisrequired,itisrecommendedthatthedevelopmenttimeisreducedortheantibody/conjugateisdilutedfurther.(DilutionofGWB-Q00248isnotrecommended).