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TheDetectX®GlutathioneS-Transferase(GST)FluorescentActivitykitisdesignedtoquantitativelymeasuretheactivityofGST.AGSTstandardisprovidedtogenerateastandardcurvefortheassay.Thekitutilizesanon-fluorescentmoleculethatisasubstratefortheGSTenzymewhichcovalentlyattachestoglutathione(GSH)toyieldahighlyfluorescentproduct.Intheendpointmode,mixingthesampleorstandardwiththesuppliedDetectionReagentandGSHyieldsafluorescentproductwhichisreadat460nminafluorescentplatereaderwithexcitationat390nm.Forratedetermination,theintensityat460nmisreadperiodicallyandplottedversustime.
TheDetectX®GlutathioneS-Transferase(GST)familyofisozymesfunctiontodetoxifyandneutralizeawidevarietyofelectrophilicmoleculesbymediatingtheirconjugationwithreducedglutathione.HumanGSTsareencodedby5genefamilies,expressinginalmostalltissuesasfourcytosolicandonemicrosomalforms.Dividingthefamilybyisoelectricpoints,thebasicalpha(pI8to11),theneutralmu(pI5to7)andacidicpi(pH<5)classesarepopulatedbyadditionalsubclasses,eachisozymedisplayingdifferentialspecificityforgivenelectrophilicmolecules.Givenitspivotalroleinamelioratingoxidativestress/damage,GSTactivityhasbeenrepeatedlyinvestigatedasabioMarkerforarthritis,asthma,COPD,andmultipleformsofcancer,aswellasanenvironmentalmarker.ExaminationofGSTisoformsandactivityinhumancancers,tumorsandtumorcelllineshasrevealedthepredominanceoftheacidicpiclass.FurThermore,thisactivityisthoughttosubstantiallycontributetotheinnateoracquiredresistanceofspecificneoplasmstoanticancertherapy.
DataReductionTemplatebyMyAssays
自1982年以来，Arbor Assays的创始人和顶尖科学家一直在研究和开发高质量的检测试剂盒。我们从小就生产FDA管制的用于人类疾病检测的体外诊断产品。1991年，当我们开始为生命科学市场生产产品时，我们意识到灵敏度，速度，便利性，质量和服务与这些产品无关。我们为您更改了。2017年1月1日，Arbor Assays通过永久员工福利信托（EBT）成为全球第一家员工拥有的生命科学公司。作为EBT，董事会确保公司为客户和员工的利益而保持独立。对于客户而言，员工拥有权意味着可以依靠公司不断进行试验，创新和提供高质量的产品。对于员工而言，所有权意味着提高工作安全性和职业满意度，而不必担心Arbor Assays将被出售给更大的开发公司。
