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This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Optimal dilution of the CD68 antibody should be determined by the researcher.
A recombinant human partial protein (amino acids 150-221) was used as the immunogen for this CD68 antibody.
Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
