PhosphodiesteraseIIPreparedfromthe1mMsodiumpyrophosphate,pH6.9,aluminageleluateofHilmoe:Biochem.PrepVIII(Meister,A.,ed.),JohnWiley&Sons,NY,NY,p.105(1961).Lyophilizedinvials.Storeat-20°C.REQUIRESSPECIALSHIPPING:ICEPACK≥1.2u/mgdwSource:BovineSpleen

I.U.B.:3.1.16.1

Spleenexonuclease(PhosphodiesteraseII)excises3"-phosphomononucleotidesfromoligonucleotideshavingafreeOHterminus.TheoptimumpHfortheenzymeis5.5usingsuccinateandphosphatebufferandpH6-7with0.1Macetatebuffer.TheenzymeisassayedusingamodificationoftheHilmoe(Biochem.Prep.,VII,(Meister,A.,ed.),JohnWileyandSons,NY,105,1961)technique.

UnitDefinition:OneUnitincreasestheabsorbanceat260nmby0.200in30minutesat37°C,pH6.5,withanRNAsubstrate.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。