Chymotrypsin,AlphaThreetimescrystallizedalphachymotrypsinwhichisanactivationproductofa3Xcrystallizedzymogen.Dialyzedagainst1mMHClandlyophilized.Storeat2-8°C.≥45unitspermgproteinSource:BovinePancreas

I.U.B.:3.4.21.1

ChymotrypsinpreferentiallycatalyzesthehydrolysisofpeptidebondsinvolvingL-isomersoftyrosine,phenylalanineandtryptophan.ItalsoreADIlyactsuponamidesandestersofsusceptIBLeaminoacids.Chymotrypsincatalyzesthehydrolysisofbondsofleucyl,methionyl,asparaginylandglutamylresidues.

StABIlity/Storage:TheenzymeisstablefordaysinsolutionatpH3.0andforyearsasadrypowderat2-8°C.Protectfrommoisture.

UnitDefinition:OneUnithydrolyzesonemicromoleofbenzoyl-L-tyrosineethylesterperminuteat25°C,pH7.8inthepresenceofcalcium.Anactivityof45unitspermgusingtheabovedefinition,istheequivalentof10,000opticaldensityor1330N.F.unitspermgusingATEEasasubstrate.

1BTEEunit=29.5USP/NFunits.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。