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4000-520-616 / 18915418616
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商品描述
**ThischainisnotrecognizedbyLUB-9,thelinearchain-specificmonoclonalantibody,norisitcleavedbyOtulin(akaGumby),thelinearchain-specificDUB.**
Awiderangeofcellularprocessesaremodulatedthroughthegenerationandattachmentofpolyubiquitin(polyUb)chainstotargetproteins. IncreasingevidencesuggeststhatpolyUbchainsjoinedthroughlinearpeptidebondsbetweentheC-terminusofaubiquitinandtheN-terminusofanotherplayimportantfunctionalroles.TheenzymemachineryresponsIBLeforthegenerationoflinearpolyUbchainshasbeentermedLUBAC,consistingofHOIL-1LandHOIP.Chainsofthesetypehaveanopenconformation,similartopolyUbK63,butwithverydistinctfunctionalproperties.LinearpolyUbchainsarecleavedbythedeubiquitylasesCYLD,USP5(IsoT),USP2andhavebeenshowntobindtomanyUBDsincludingNEMOandTrABIn-n(3xnzf).ThesespecificchainsarenotcleavedbythelinearspecificdeubiquitylaseOtulinandhaveapplicationsasacontrol.
RecombinantlinearchainsofdefinedlengthexpressedinE.coliandpurifiedtohomogeneity.AmidelinkagesjointheN-andC-terminusofeachubiquitinmoleculetoeachother.ThismoleculeisHIS-taggedattheN-terminusofthemostdistalubiquitin.
Info
Source | HumanRecombinant |
State | Liquid |
Molecularweight | 17,786.2Da |
AffinityTag | His6 |
Buffer | 20mMTris,pH7.5,0.15MNaCl,1mMEDTA |
Storage | -80°C,avoidcyclesoffreeze/thaw |
Stability | 1yearunderrecommendedstorageconditions |
Applications
- Investigationofbindinginteractionsthroughpull-downstudies.
- Determiningpotentialdeubiquitylaseactivitytowardslinearchains.
- Pleasenote:TheselinearubiquitinchainshavehadtheMethionineresiduebetweeneachubiquitinmoietygeneticallyremoved,i.e.Gly76isfuseddirectlytoGln2. Asaresult,theyarenotrecognizedbyLUB-9,thelinearchain-specificmonoclonalantibody,noraretheycleavedbyOtulin(akaGumby),thelinearchain-specificDUB.
Documents
Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用
