ForremovalofSUMOpro3tagsfromfusionproteinsexpressedfromthefollowingSUMOpro3expressionsystems:(Cat.#s: 1010A, 1016, 1010K, 1011, 2150, 2151).SUMOProtease2,ahighlyactiveandrobustrecombinantprotease,cleaveshSUMO3fromrecombinantfusionproteins.Unlikethrombin,EK,orTEVprotease,whichrecognizeshort,linearsequences,SUMOProtease2recognizesthetertiarystructureofhuSUMO3.Asaresult,SUMOProtease2willnotcleavewithinthefusedproteinofinterest.

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Info

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pfam	PeptidaseC-48
Molecularweight	28kDa
Fusiontag	His6
Source	E.coli
Purity	>95%
Physicalstate	10U/µLin50mMHepes,pH7.5,150mMNaCl,10%glycerol
Activity	>105Unit/mgcore 1UofSUMOProtease1cleaves>90%of100µgSUMOProtease1ControlProteinin1hour(30°C)
Storage/StABIlity	Storeat-80°C,avoidingrepeatedfreeze-thawcycles.Stableforuptooneyearundertheseconditions.

Applications

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• DigestionandremovalofSUMOprotagsfromrecombinantfusionproteinsmadewithourSUMOproExpressionsystems (Cat.#s: 1010A, 1016, 1010K, 1011, 2150, 2151)

Benefits

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• SUMOProtease1cleavesconsistentlyoverabroadrangeoftemperature(30°Cisoptimal),pH[5.5-9.5],andionicstrength.SUMOProtease1containsapolyhistidinetagattheN-terminus,makingiteasytoremovefromthecleavagereactionbyaffinitychromatography.

Documents

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• Datasheet
• LimiteduseofLabelLicense

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用