Description
AmajorlimitationofPolymeraseChainReaction(PCR)-basedresearchanddiagnosticandforensictestsistheinhibitionofDNApolymerasesbymanysubstanceslikeasanticoagulants(e.g.,heparin,EDTA,sodiumcitrate)andhemegroup,proteins,polysaccharides,celldebris,andchargedinorganiccompounds,etc.Therefore,DNAisusuallyextractedfromspecimenaccordingtophenol-chloroformmethodorbyanalternative‘silica-membranefilter’method.However,thisistimeconsumingandlaborintensiveandthereisalsoariskofintroducingcontaminationduringthepreparation. EzWayDirectFPCRallowsdirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture.EzWayDirectFBuffercontainstheproperamountsofbuffer,MgCl2,dNTPsandthecomponentsthatfacilitatedirectPCRamplification,buthotstartenzymeissuppliedwithseparatetubefortheextendedactivity
Specification
•DirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture
Components
•EzWayDirectFBufferMix100T(25uLPCR) •HotTaq-fx(5units/uL) •25xInhibitorcocktail
Platform
•AppliedBiosystems310GeneticAnalyzerfilledwithPOP-4&36cmcapillaries •ABIPRISM®3100/3100-AvantGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •AppliedBiosystems3130/3130xlGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •GeneAmp®PCRSystem9600/9700/Veriti
Applications
Humanidentificationwithaforensicsample
Citation

Koma BioTech的HLA等位基因与许多疾病有关，并且与许多类型的免疫反应有关。HLA类型的确定将揭示测试药物功效和对多种疾病的反应的重要信息。KOMABIOTECH通过采用Luminex xMAP技术的SSO探针杂交（SSOP）提供HLA测试。■ 特征：•根据研究的需要，HLA类型的确定可以基于单个抗原的分析，也可以跨多个抗原进行•场所提供更详细和全面的报告。•该服务仅用于研发/非诊断目的。•HLA输入执行了4位（“高”）分辨率。涵盖的基因：HLA-A，HLA-B，HLA-C，HLA-DRB1，HLA-DPB1，HLA-DQB1