Description	Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Batch No.	See product label
Unit size	50 µl
Antigen	Purified bovine NF-H. Bovine intermediate filaments were prepared from spinal cords and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis.
Antibody Type	Antiserum
Other Names	NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession	P12036 NFH_HUMAN;
Produced in	Rabbit
Applications	Western Blotting (WB), ELISA and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa.
Antibody Against	Neurofilament Heavy, phosphorylated
Cross-reactivity	Rat. Predicted to react with other mammals due to sequence homology.
Blast URL	Click here
Form	Lyophilised
Appearance	White powder
Reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

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