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蚂蚁淘在线 / 品牌中心 / Alomone / 芦荟素/抗人腺苷A2A受体（细胞外）抗体/AAR-007/25µl

芦荟素/抗人腺苷A2A受体（细胞外）抗体/AAR-007/25µl

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￥4940.00

货号：AAR-007-25µl

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商品描述

Cat#:AAR-007

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h

Immunogen

PeptideCGQPKEGKNHSQG,correspondingtoaminoacidresidues146-158ofhumanadenosine A_2A receptor(AccessionP29274).2^ndextracellularloop.

Anti-Human Adenosine A2AReceptor (extracellular) Antibody -

Accession(Uniprot)NumberP29274

GeneID135

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHumanonly.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN_3.

SpecificityWillnotrecognizeratormousesamples.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ifc,wb

Mayalsoworkin:ic,ih,ip,lci

Westernblot

WesternblotanalysisofhumanHL-60promyelocyticleukemiacelllinelysate(lanes1and3)andhumanJurkatT-cellleukemiacelllinelysate(lanes2and4):

1,2.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(1:200).
3,4.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Indirectflowcytometry

IndirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___ Cells.
^___Cells+goat-anti-rabbit-FITC.
^___Cells+ Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(2.5µg)+goat-anti-rabbit-FITC.

IndirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___ Cells.
^___Cells+goat-anti-rabbit-FITC.
^___Cells+ Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(2.5µg)+goat-anti-rabbit-FITC.

References

Okusa,M.D.(2002)Am.J.Physiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Cunha,R.A.(2001)NeuRochem.Int.38,107.
Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
Canals,M.etal.(2005)J.Neurochem.92,337.
Ohta,A.andSitkovsky,M.(2001)Nature414,916.
Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart^1,2. Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors): AdenosineA₁ Receptor (A₁AR),AdenosineA_2A Receptor(A_2AAR), AdenosineA_2B Receptor (A_2BAR),and AdenosineA₃ Receptor (A₃AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH₂ terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportant forbindingGproteins.^1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs^1-3.

Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A_2AARandtheinhibitoryA₁AR.⁴ A_2AARandA₁ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A_2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA₁ARiswidelydistributedthroughouttheCNS^5,6.

A_2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.⁷ ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA_2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.⁷ ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA_2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues⁸.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:24/03/2019

Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody (#AAR-007)isahighlyspecificantibodydirectedagainstanepitopeofhumanprotein.Theantibodycanbeusedinwesternblotandlivecellflowcytometryapplications.IthasbeendesignedtorecognizeA_2ARfromhumansamplesonly.Theantibodywillnotrecognizethereceptorfrommouseorratsamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

WesternblotanalysisofhumanHL-60promyelocyticleukemiacelllinelysate(lanes1and3)andhumanJurkatT-cellleukemiacelllinelysate(lanes2and4):

1,2.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(1:200).
3,4.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Alomone Labs是您离子通道研究的合作伙伴，是离子通道，GPCR和神经信号工具的领先供应商。我们专门生产基础科学研究中使用的一抗，小分子，蛋白质和肽。Alomone成立于1989年，现已成为全世界科学家使用的最全面的离子通道研究工具来源。Alomone的所有产品均经过生物测定验证，并以冻干粉形式提供，因此您可以对它们的生物活性和长保质期充满信心。立即索取信息 Alomone公司作为世界上***的离子通道专业供应商，它的离子通道抗体是世界上*全的、质量也非常好！目前被世界各地的科学家广泛的使用. Alomone Labs Alomone 公司是一家位于耶路撒冷的以色列著名的生物科技公司，专著于提供生命科学细胞生物学试剂和服务。Alomone 公司作为世界上最著名的离子通道专业供应商，它的离子通道抗体是世界上最全的、质量也非常好！目前被世界各地的科学家广泛的使用。试剂

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芦荟素/抗人腺苷A2A受体（细胞外）抗体/AAR-007/25µl

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