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蚂蚁淘在线 / 品牌中心 / Alomone / 阿洛蒙/抗腺苷A2A受体抗体/AAR-002/50µl

阿洛蒙/抗腺苷A2A受体抗体/AAR-002/50µl

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￥7340.00

货号：AAR-002-50µl

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商品描述

Cat#:AAR-002

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)RQLKQMESQPLPGER,correspondingtoaminoacidresidues201-215ofmouseadenosineA_2A receptor(AccessionQ60613).3^rdintracellularloop.

Accession(Uniprot)NumberQ60613

GeneID11540

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat,human,andcanis–identical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.75mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ih,wb

Mayalsoworkin:ifc,ip

Westernblot

Westernblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:

1,2,5.Anti-Adenosine A_2AReceptor Antibody(#AAR-002),(1:200).
3,4,6.Anti-AdenosineA_2AReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

ExpressionofAdenosine A_2A Receptor in mousediagonalband

ImmunohistochemicalstainingofmousediagonalbandusingAnti-Adenosine A_2A ReceptorAntibody(#AAR-002).A2aR (green)appearsinthebroca ofindividualneurons(arrows)andinneuropil(asterisk). DAPIisusedasthecounterstain.

ExpressionofAdenosine A_2A Receptor inratlung

ImmunohistochemicalstainingofparaffinemeddedratlungsectionsusingAnti-Adenosine A_2A Receptor Antibody(#AAR-002),(1:50).A2aRisexpressedintherespiratoryepitheliumofthebronchioli(Br).Notethatsmoothmuscleandendotheliuminbloodvesselsarenegative.Hematoxilinisusedasthecounterstain.

Immunocytochemistry

Ratneurospheres(Benito-Munoz,M. etal. (2016) Glia 64, 1465.).

References

Okusa,M.D.(2002)Am.J.Physiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Cunha,R.A.(2001)NeuRochem.Int.38,107.
Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
Canals,M.etal.(2005)J.Neurochem.92,337.
Ohta,A.andSitkovsky,M.(2001)Nature414,916.
Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.^1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA₁ Receptor(A₁AR),AdenosineA_2A Receptor(A_2AAR),AdenosineA_2B Receptor(A_2BAR),andAdenosineA₃ Receptor(A₃AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH₂ terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.^1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.^1-3

Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A_2AARandtheinhibitoryA₁AR.⁴ A_2AARandA₁ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A_2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA₁ARiswidelydistributedthroughouttheCNS.^5,6

A_2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.⁷ ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA_2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.⁷ ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA_2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues.⁸

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:17/02/2019

Anti-Adenosine A_2A ReceptorAntibody(#AAR-002)isahighlyspecificantibodydirectedagainstanepitopeofmouseprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeA2aRfromhuman,rat,andmousesamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:

1,2,5.Anti-Adenosine A_2AReceptor Antibody(#AAR-002),(1:200).
3,4,6.Anti-AdenosineA_2AReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

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阿洛蒙/抗腺苷A2A受体抗体/AAR-002/50µl

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