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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Background:
TheadenovirusE1A-associatedcellularp300transcriptionalco-activatorproteinfunctionsasahistoneacetyltransferasethatregulatestranscriptionviachromatinremodelingandisimportantintheprocessesofcellproliferationanddifferentiation.p300(alsoknownasKAT3B)acetylatesallfourcorehistonesinnucleosomes,aswellasnon-histonetargets.p300mediatescAMP-generegulationbybindingspecificallytophosphorylatedCREBprotein.p300hasalsobeenidentifiedasaco-activatorofHIF1A(hypoxia-inducIBLefactor1alpha),whichplaysaroleinthestimulationofhypoxia-inducedgenessuchasVEGF.DefectsinthisgeneareacauseofRubinstein-TaybisyndromeanditsdysfunctionislinkedtoanumberofhumandiseasesincludingParkinson’s,Huntington’s,andAlzheimer’sdiseases.p300mayalsoplayaroleinepithelialcancer.
Description:
Thep300ChemiluminescentAssayKitisanenzyme-linkedimmunosorbentassay(ELISA)designedtoscreenforinhibitorsofp300.TheP300ChemiluminescentAssayKitisbasedonthep300enzymetransferringanacetylgroupfromanacetyldonor(acetylCoA)toahistonesubstrate.Theacetylatedhistoneisrecognizedbyahighlyspecificprimaryantibody,followedbyanHRP-labeledsecondaryantibody.ThechemiluminescenceproducedbyHRPcanbemeasuredusingachemiluminescencereader.
UniProt
Q09472
Synonym(s):
EP300,KAT3B,RSTS2,E1Abindingprotein
SuppliedAs:
Kitcomesinaconvenientformat,witha96-wellplateprecoatedwithNeutravidinandHistonepeptide,andallthereagentsnecessaryfor96chemiluminescentP300activitymeasurements.Inaddition,thekitincludespurifiedp300foruseasapositivecon
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt.
Format:
COMPONENTS:

Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymeactivityandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Ravindra,K.C.,etal.(2012).ChemResToxicol.25(2):337–347.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Acetyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.


